Rabbit CYB5R3 Polyclonal Antibody | anti-CYB5R3 antibody

CYB5R3 Polyclonal Antibody

Cat. Num. AAA9128660

• Gene Names: CYB5R3; B5R; DIA1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: CYB5R3; Polyclonal Antibody; CYB5R3 Polyclonal Antibody; anti-CYB5R3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 334

• Applicable Applications for anti-CYB5R3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB 1:500 - 1:2000; IHC 1:20 - 1:200; IF 1:10 - 1:100; IP 1:20 - 1:50
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CYB5R3
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CYB5R3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Related Product Information for anti-CYB5R3 antibody

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.

NCBI and Uniprot Product Information

• NCBI GI #: 4503327
• NCBI GeneID: 1727
• NCBI Accession #: NP_000389.1
• NCBI GenBank Nucleotide #: NM_000398.6
• UniProt Accession #: P00387; O75675; Q8TDL8; Q8WTS8; Q9UEN4; Q9UEN5; Q9UL55; Q9UL56; B1AHF2; B7Z7L3
• Molecular Weight: 34kDa
• NCBI Official Full Name: NADH-cytochrome b5 reductase 3 isoform 1
• NCBI Official Synonym Full Names: cytochrome b5 reductase 3
• NCBI Official Symbol: CYB5R3
• NCBI Official Synonym Symbols: B5R; DIA1
• NCBI Protein Information: NADH-cytochrome b5 reductase 3
• UniProt Protein Name: NADH-cytochrome b5 reductase 3
• Protein Family: NADH-cytochrome b5 reductase
• UniProt Gene Name: CYB5R3
• UniProt Synonym Gene Names: DIA1; B5R; Cytochrome b5 reductase
• UniProt Entry Name: NB5R3_HUMAN

NCBI Description

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]

Uniprot Description

CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 1.6.2.2; Oxidoreductase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; endoplasmic reticulum; hemoglobin complex; mitochondrial inner membrane; cytoplasm; lipid particle

Molecular Function: FAD binding; cytochrome-b5 reductase activity; ADP binding; NAD binding; AMP binding

Biological Process: vitamin metabolic process; blood circulation; L-ascorbic acid metabolic process; cholesterol biosynthetic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Due To Deficiency Of Methemoglobin Reductase