Western Blot (WB)
(Western blot analysis of CYB561D1 expression in Hela (A), NIH3T3 (B) whole cell lysates.)
Rabbit anti-Human, Mouse CYB561D1 Polyclonal Antibody | anti-CYB561D1 antibody
Anti-CYB561D1 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
CYB561D1; Polyclonal Antibody; Anti-CYB561D1 Antibody; Cytochrome b561 domain-containing protein 1; anti-CYB561D1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CYB561D1 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
229
Applicable Applications for anti-CYB561D1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CYB561D1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of CYB561D1 expression in Hela (A), NIH3T3 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CYB561D1 expression in Hela (A), NIH3T3 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of CYB561D1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of CYB561D1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-CYB561D1 antibody
Rabbit polyclonal antibody to CYB561D1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,314 Da
NCBI Official Full Name
Cytochrome b561 domain-containing protein 1
NCBI Official Synonym Full Names
cytochrome b561 family member D1
NCBI Official Symbol
CYB561D1
NCBI Protein Information
cytochrome b561 domain-containing protein 1
UniProt Protein Name
Cytochrome b561 domain-containing protein 1
Protein Family
UniProt Gene Name
CYB561D1
UniProt Entry Name
C56D1_HUMAN
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Product Notes
The CYB561D1 cyb561d1 (Catalog #AAA8242811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYB561D1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYB561D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the CYB561D1 cyb561d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYB561D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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