Rabbit anti-Human CXCR5 Polyclonal Antibody | anti-CXCR5 antibody

CXCR5, NT (CXCR5, BLR1, MDR15, C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, CD185) (AP)

Cat. Num. AAA6290243

• Gene Names: CXCR5; BLR1; CD185; MDR15; MGC117347
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CXCR5; Polyclonal Antibody; NT (CXCR5; BLR1; MDR15; C-X-C chemokine receptor type 5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15; CD185) (AP); CD185; anti-CXCR5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CXCR5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CXCR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CXCR5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CXCR5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CXCR5 antibody detected the CXCR5 protein (arrow).)

Related Product Information for anti-CXCR5 antibody

This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein-coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist.

Product Categories/Family for anti-CXCR5 antibody

Antibodies; CD Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Lee, H.T., et al. J. Rheumatol. 37(1):45-52(2010). El Haibi, C.P., et al. Mol. Cancer 9, 85 (2010): Singh, S., et al. Int. J. Cancer 125(10):2288-2295(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14589869
• NCBI GeneID: 643
• NCBI Accession #: NP_116743.1
• NCBI GenBank Nucleotide #: NM_032966.2
• UniProt Accession #: P32302; Q14811; Q2YD84
• Molecular Weight: 41,955 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 5 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 5
• NCBI Official Symbol: CXCR5
• NCBI Official Synonym Symbols: BLR1; CD185; MDR15; MGC117347
• NCBI Protein Information: C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; MDR-15; OTTHUMP00000232687; monocyte-derived receptor 15; Burkitt lymphoma receptor 1, GTP-binding protein; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)
• UniProt Protein Name: C-X-C chemokine receptor type 5
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR5
• UniProt Synonym Gene Names: BLR1; MDR15; CXC-R5; CXCR-5; MDR-15
• UniProt Entry Name: CXCR5_HUMAN

Uniprot Description

CXCR5: Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; C-X-C chemokine receptor activity

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; B cell activation; immune response; cell motility; chemotaxis; lymph node development