Western Blot (WB)
(Anti-CXCR4 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:46 kDa)
Rabbit anti-Human CXCR4/CD184 Polyclonal Antibody | anti-CXCR4 antibody
Anti-CXCR4/CD184 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CXCR4/CD184; Polyclonal Antibody; Anti-CXCR4/CD184 Antibody; Rabbit Polyclonal; CXCR4/CD184 Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X-C motif) receptor 4; Anti-CD184 Antibody; Anti-D2S201E Antibody; Anti-FB22 Antibody; Anti-HM89 Antibody; Anti-HSY3RR Antibody; Anti-LAP-3 Antibody; Anti-LAP3 Antibody; Anti-LCR1 Antibody; Anti-LESTR Antibody; Anti-NPY3R Antibody; Anti-NPYR Antibody; Anti-NPYRL Antibody; Anti-NPYY3R Antibody; Anti-WHIM Antibody; Anti-WHIMS Antibody; anti-CXCR4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CXCR4/CD184
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CXCR4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 0.2-1uL/mg of lysate
IP: 0.2-1uL/mg of lysate
Immunogen
E Coli-derived Human CXCR4/CD184 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CXCR4/CD184 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CXCR4 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:46 kDa)
Western Blot (WB)
(Anti-CXCR4 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:46 kDa)
Immunoprecipitation (IP)
(CXCR4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-CXCR4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CXCR4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :45 kDa)
Immunoprecipitation (IP)
(CXCR4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 1 uL anti-CXCR4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CXCR4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :45 kDa)
Product Categories/Family for anti-CXCR4 antibody
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Product Notes
The CXCR4 (Catalog #AAA8110507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CXCR4/CD184 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CXCR4/CD184 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the CXCR4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR4/CD184, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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