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Western Blot (WB) (Western Blot analysis of various cells using Connexin 25 Polyclonal Antibody diluted at 1?500)

Rabbit anti-Human CXB7 Polyclonal Antibody | anti-CXB7 antibody

CXB7 polyclonal antibody

Gene Names
GJB7; CX25; bA136M9.1; connexin25
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CXB7; Polyclonal Antibody; CXB7 polyclonal antibody; GJB7; CX25; Gap junction beta-7 protein; Connexin-25; Cx25; anti-CXB7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Connexin 25 Polyclonal Antibody detects endogenous levels of Connexin 25 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-CXB7 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
ELISA: 1/10,000
Immunogen
The antiserum was produced against synthesized peptide derived from human GJB7. AA range: 21-70
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using Connexin 25 Polyclonal Antibody diluted at 1?500)

Western Blot (WB) (Western Blot analysis of various cells using Connexin 25 Polyclonal Antibody diluted at 1?500)

Western Blot (WB)

(Western blot analysis of lysates from HT-29 cells, using GJB7 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from HT-29 cells, using GJB7 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of the lysates from HeLa cells using GJB7 antibody.)

Western Blot (WB) (Western blot analysis of the lysates from HeLa cells using GJB7 antibody.)
Related Product Information for anti-CXB7 antibody
Background: gap junction protein beta 7(GJB7) Homo sapiens. Connexins, such as GJB7, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,860 Da
NCBI Official Full Name
gap junction beta-7 protein
NCBI Official Synonym Full Names
gap junction protein, beta 7, 25kDa
NCBI Official Symbol
GJB7
NCBI Official Synonym Symbols
CX25; bA136M9.1; connexin25
NCBI Protein Information
gap junction beta-7 protein; connexin 25; connexin-25
UniProt Protein Name
Gap junction beta-7 protein
UniProt Gene Name
GJB7
UniProt Synonym Gene Names
CX25; Cx25
UniProt Entry Name
CXB7_HUMAN

NCBI Description

Connexins, such as GJB7, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]

Uniprot Description

GJB7: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: connexon complex; integral to membrane

Biological Process: cell communication

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Product Notes

The CXB7 gjb7 (Catalog #AAA3001852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CXB7 polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CXB7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1/500-1/2000 ELISA: 1/10,000. Researchers should empirically determine the suitability of the CXB7 gjb7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXB7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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