Goat Cullin 3 Polyclonal Antibody | anti-CUL3 antibody

Goat anti-Cullin 3 Antibody

Cat. Num. AAA423310

• Gene Names: CUL3; CUL-3; PHA2E
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Cullin 3; Polyclonal Antibody; Goat anti-Cullin 3 Antibody; CUL3; cullin 3; KIAA0617; CUL3 antibody; cullin 3 antibody; KIAA0617 antibody; anti-CUL3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: AQVTGSNTRKHILQ
• Sequence Length: 768

• Applicable Applications for anti-CUL3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 88.9kDa according to NP_003581.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-AQVTGSNTRKHILQ, from the internal region of the protein sequence according to NP_003581.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503165
• NCBI GeneID: 8452
• NCBI Accession #: NP_003581.1
• NCBI GenBank Nucleotide #: NM_003590.4
• Molecular Weight: 81,093 Da
• NCBI Official Full Name: cullin-3 isoform 1
• NCBI Official Synonym Full Names: cullin 3
• NCBI Official Symbol: CUL3
• NCBI Official Synonym Symbols: CUL-3; PHA2E
• NCBI Protein Information: cullin-3
• UniProt Protein Name: Cullin-3
• Protein Family: Cullin
• UniProt Gene Name: CUL3
• UniProt Synonym Gene Names: KIAA0617; CUL-3
• UniProt Entry Name: CUL3_HUMAN

NCBI Description

This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

CUL3: a core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. SPOP is involved in ubiquitination of BMI1, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31A or -B. BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Forms neddylation-dependent homodimers. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein acting as both, adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1, RNF7, CYCE and CAND1. Part of the BCR(SPOP) containing SPOP. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KBTBD10) complex containing KBTBD10. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL3 and RBX1 (Probable). Part of the BCR(ENC1) complex containing ENC1. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1P1, BTBD1. Part of a complex that contains CUL3, RBX1 and GAN. Interacts (via BTB domain) with KLHL17; the interaction regulates surface GRIK2 expression. Widely expressed. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Cell cycle regulation; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2q36.2

Cellular Component: cytosol; Golgi membrane; membrane; nucleoplasm; polar microtubule

Molecular Function: cyclin binding; POZ domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cell cycle arrest; cell migration; cell projection organization and biogenesis; COPII coating of Golgi vesicle; cyclin catabolic process; embryonic cleavage; ER to Golgi vesicle-mediated transport; G1/S transition of mitotic cell cycle; gastrulation; integrin-mediated signaling pathway; MAPKKK cascade; mitotic metaphase plate congression; negative regulation of Rho protein signal transduction; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of cytokinesis; positive regulation of mitotic metaphase/anaphase transition; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; stem cell division; stress fiber formation; trophectodermal cellular morphogenesis; Wnt receptor signaling pathway

Disease: Pseudohypoaldosteronism, Type Iie