Rabbit anti-Human, Mouse Cul7 Polyclonal Antibody | anti-Cul7 antibody

Anti-Human Cul7 (C-Terminal Specific) Antibody

Cat. Num. AAA4157829

• Gene Names: CUL7; 3M1; CUL-7; KIAA0076; dJ20C7.5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified
• Synonyms: Cul7; Polyclonal Antibody; Anti-Human Cul7 (C-Terminal Specific) Antibody; Human Cul7; anti-Cul7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.
• Purity/Purification: Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 1698

• Applicable Applications for anti-Cul7 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: This antibody reacts with human and mouse Cul7 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and also immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems. A 191.2 kDa band corresponding to human Cul7 is detected. Most cell lines expressing Cul7 can be used as a positive control.
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled to KLH.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-Cul7 antibody

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

NCBI and Uniprot Product Information

• NCBI GI #: 41872646
• NCBI GeneID: 9820
• NCBI GenBank Nucleotide #: NP_055595.2
• UniProt Accession #: Q14999
• NCBI Official Full Name: cullin-7 isoform 2
• NCBI Official Synonym Full Names: cullin 7
• NCBI Official Symbol: CUL7
• NCBI Official Synonym Symbols: 3M1; CUL-7; KIAA0076; dJ20C7.5
• NCBI Protein Information: cullin-7
• UniProt Protein Name: Cullin-7
• Protein Family: Cullin
• UniProt Gene Name: CUL7
• UniProt Synonym Gene Names: KIAA0076; CUL-7
• UniProt Entry Name: CUL7_HUMAN

NCBI Description

The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

CUL7: Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. Seems not to promote polyubiquitination and proteasomal degradation of TP53. In vitro, complexes of CUL7 with either CUL9 or FBXW8 or TP53 contain E3 ubiquitin-protein ligase activity. Defects in CUL7 are the cause of 3M syndrome type 1 (3M1). An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies. Belongs to the cullin family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; viral reproduction; unfolded protein response; protein ubiquitination; microtubule cytoskeleton organization and biogenesis; positive regulation of dendrite morphogenesis; proteolysis; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cytokinesis after mitosis; epithelial to mesenchymal transition; regulation of mitosis; vasculogenesis; Golgi organization and biogenesis; placenta development

Disease: Three M Syndrome 1