Rabbit anti-Human CUL4B Polyclonal Antibody | anti-CUL4B antibody

CUL4B, ID (CUL4B, KIAA0695, Cullin-4B) (PE)

Cat. Num. AAA6290088

• Gene Names: Cul4b; CUL-4B; AA409770; mKIAA0695; 2700050M05Rik
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CUL4B; Polyclonal Antibody; ID (CUL4B; KIAA0695; Cullin-4B) (PE); Cullin-4B; anti-CUL4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CUL4B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CUL4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-260 amino acids from the Central region of human CUL4B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CUL4B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate. CUL4B Antibody (Center) (arrow) was detected using the purified Pab.)

Related Product Information for anti-CUL4B antibody

This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-CUL4B antibody

Antibodies; Cullin

References

Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010). Abbas, T., et al. Mol. Cell 40(1):9-21(2010). Kerzendorfer, C., et al. Hum. Mol. Genet. 19(7):1324-1334(2010). Gascoin-Lachambre, G., et al. Placenta 31(2):151-157(2010). Badura-Stronka, M., et al. Clin. Genet. 77(2):141-144(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 298351634
• NCBI GeneID: 72584
• UniProt Accession #: Q13620; Q3TU30; Q8BSL3; Q8CHD6; Q91YZ7; Q99KS9; Q9CZM5; A2A432
• Molecular Weight: 107,391 Da
• NCBI Official Full Name: Cullin-4B
• NCBI Official Synonym Full Names: cullin 4B
• NCBI Official Symbol: Cul4b
• NCBI Official Synonym Symbols: CUL-4B; AA409770; mKIAA0695; 2700050M05Rik
• NCBI Protein Information: cullin-4B
• UniProt Protein Name: Cullin-4B
• Protein Family: Cullin
• UniProt Gene Name: CUL4B
• UniProt Synonym Gene Names: KIAA0695; CUL-4B
• UniProt Entry Name: CUL4B_HUMAN

Uniprot Description

CUL4B iso1: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. Defects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC); also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: nucleoplasm; cytoplasm; plasma membrane

Molecular Function: protein binding; ubiquitin protein ligase binding; damaged DNA binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; cell cycle

Disease: Mental Retardation, X-linked, With Short Stature, Hypogonadism, And Abnormal Gait