Rabbit CUL1 Polyclonal Antibody | anti-CUL1 antibody

CUL1 (Cullin-1, MGC149834, MGC149835) (Biotin)

Cat. Num. AAA6413089

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: CUL1; Polyclonal Antibody; CUL1 (Cullin-1; MGC149834; MGC149835) (Biotin); anti-CUL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes CUL1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CUL1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human CUL1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using CUL1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human stomach cancer using CUL1 antibody at dilution of 1:200 (200x lens).)

Product Categories/Family for anti-CUL1 antibody

Antibodies; Cullin

NCBI and Uniprot Product Information

• NCBI GI #: 32307161
• NCBI GeneID: 8454
• NCBI Accession #: NP_003583.2
• NCBI GenBank Nucleotide #: NM_003592.2
• UniProt Accession #: Q13616; O60719; Q08AL6; Q8IYW1; D3DWG3
• Molecular Weight: 89,679 Da
• NCBI Official Full Name: cullin-1
• NCBI Official Synonym Full Names: cullin 1
• NCBI Official Symbol: CUL1
• NCBI Protein Information: cullin-1; CUL-1
• UniProt Protein Name: Cullin-1
• Protein Family: Cullin
• UniProt Gene Name: CUL1
• UniProt Synonym Gene Names: CUL-1
• UniProt Entry Name: CUL1_HUMAN

Uniprot Description

CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; cell cycle arrest; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle