Rabbit anti-Human CTSK Polyclonal Antibody | anti-CTSK antibody

CTSK (ID R222) (Cathepsin K, Cathepsin O, Cathepsin X, Cathepsin O2, CTSO2, CTSO) (MaxLight 490)

Cat. Num. AAA6467725

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CTSK; Polyclonal Antibody; CTSK (ID R222) (Cathepsin K; Cathepsin O; Cathepsin X; Cathepsin O2; CTSO2; CTSO) (MaxLight 490); anti-CTSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTSK.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CTSK antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: FLISA: 1:1,000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; IHC: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CTSK (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma with CTSK Antibody (Center R222), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ZR-75-1 cells using CTSK Antibody (Center R222)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center R222). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTSK gene (Lane 2).)

Product Categories/Family for anti-CTSK antibody

Antibodies; Cathepsin

References

1. Lendeckel, U., Neurochem. Int. 54(7), 410-417 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503151
• NCBI GeneID: 1513
• NCBI Accession #: NP_000387
• NCBI GenBank Nucleotide #: NM_000396
• UniProt Accession #: P43235
• Molecular Weight: 37kDa
• NCBI Official Full Name: cathepsin K preproprotein
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.38

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; protein binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: extracellular matrix disassembly; collagen catabolic process; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis