Western Blot (WB)
(Western blot analysis of Hepg2 whole cell lysates, using CTSA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit CTSA Polyclonal Antibody | anti-CTSA antibody
CTSA Antibody
Gene Names
CTSA; GSL; GLB2; NGBE; PPCA; PPGB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CTSA; Polyclonal Antibody; CTSA Antibody; beta galactosidase 2; BETA GALACTOSIDASE PROTECTIVE PROTEIN; beta-galactosidase 2; beta-galactosidase protective protein; betagalactosidase 2; Carboxypeptidase C; Carboxypeptidase L; carboxypeptidase Y-like kininase; Cathepsin A; Ctsa; deamidase; EC 3.4.16.5; Glactosialidosis; GLB2; Goldberg Syndrome; GSL; lysosomal carboxypeptidase A; Lysosomal protective protein 20 kDa chain; Lysosomal protective protein; Lysosomal protective protein deficiency; NEURAMINIDASE BETA GALACTOSIDASE EXPRESSION; NGBE; Neuraminidase deficiency with beta-galactosidase deficiency; OTTHUMP00000031778; OTTHUMP00000031781; PPCA; PPCA deficiency; PPGB; PPGB_HUMAN; Protective protein cathepsin A; Protective protein for beta galactosidase; Protective protein for beta-galactosidase; Protective protein/cathepsin A deficiency; urinary kininase; anti-CTSA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CTSA antibody detects endogenous levels of total CTSA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
498
Applicable Applications for anti-CTSA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human CTSA
Subcellular Location
Lysosome.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Hepg2 whole cell lysates, using CTSA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Hepg2 whole cell lysates, using CTSA Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9608018 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9608018 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CTSA antibody
Description: This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Function: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Subunit Structure: Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.
Similarity: Belongs to the peptidase S10 family.
Function: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Subunit Structure: Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.
Similarity: Belongs to the peptidase S10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
lysosomal protective protein isoform a
NCBI Official Synonym Full Names
cathepsin A
NCBI Official Symbol
CTSA
NCBI Official Synonym Symbols
GSL; GLB2; NGBE; PPCA; PPGB
NCBI Protein Information
lysosomal protective protein
UniProt Protein Name
Lysosomal protective protein
Protein Family
UniProt Gene Name
CTSA
UniProt Synonym Gene Names
PPGB; PPCA
NCBI Description
This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]
Uniprot Description
Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Research Articles on CTSA
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Product Notes
The CTSA ctsa (Catalog #AAA9608018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CTSA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CTSA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CTSA ctsa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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