Rabbit anti-Human, Mouse CTNNA1 Polyclonal Antibody | anti-CTNNA1 antibody

CTNNA1, phosphorylated (Ser641) (Catenin alpha-1, Catenin alpha1, Alpha E-catenin, Cadherin-associated Protein, Renal Carcinoma Antigen NY-REN-13)

Cat. Num. AAA626856

• Gene Names: CTNNA1; CAP102; FLJ36832; FLJ52416
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CTNNA1; Polyclonal Antibody; phosphorylated (Ser641) (Catenin alpha-1; Catenin alpha1; Alpha E-catenin; Cadherin-associated Protein; Renal Carcinoma Antigen NY-REN-13); Anti -CTNNA1; anti-CTNNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Catenin alpha1 when phosphorylated at serine 641. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CTNNA1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic phosphopeptide corresponding to human a-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CTNNA1 antibody

Three catenin proteins, a, b and y, bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion. Not only associated with E-cadherin, a-catenin was also shown to associate with other members of the cadherin family, such as Ncadherin and P-cadherin. b-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. b-catenin has also been found in complexes with the tumor suppressor protein APC. y-catenin, also known as plakoglobin, is a protein that binds with a-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTP associates with catenin/cadherin complexes and may regulate complex signaling.

Product Categories/Family for anti-CTNNA1 antibody

Antibodies; Abs to Catenin

NCBI and Uniprot Product Information

• NCBI GI #: 55770844
• NCBI GeneID: 1495
• NCBI Accession #: NP_001894.2
• NCBI GenBank Nucleotide #: NM_001903.2
• UniProt Accession #: P35221; Q12795; Q8N1C0
• Molecular Weight: 100,071 Da
• NCBI Official Full Name: catenin alpha-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), alpha 1, 102kDa
• NCBI Official Symbol: CTNNA1
• NCBI Official Synonym Symbols: CAP102; FLJ36832; FLJ52416
• NCBI Protein Information: catenin alpha-1; alpha-catenin; alphaE-catenin; alpha E-catenin; alpha-E-catenin; OTTHUMP00000224141; OTTHUMP00000224147; renal carcinoma antigen NY-REN-13; cadherin-associated protein,102kDa
• UniProt Protein Name: Catenin alpha-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNA1
• UniProt Entry Name: CTNA1_HUMAN

Uniprot Description

CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: focal adhesion; lamellipodium; plasma membrane; acrosome; intercellular junction; catenin complex; zonula adherens; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; cadherin binding; gamma-catenin binding; beta-catenin binding; structural molecule activity; vinculin binding

Biological Process: intercellular junction assembly and maintenance; apical junction assembly; gap junction assembly; protein heterooligomerization; axon regeneration; male gonad development; establishment and/or maintenance of cell polarity; actin filament organization; odontogenesis of dentine-containing teeth; muscle cell differentiation; negative regulation of neuroblast proliferation; ovarian follicle development; response to estrogen stimulus; positive regulation of muscle cell differentiation; positive regulation of smoothened signaling pathway; vascular endothelial growth factor receptor signaling pathway; cell adhesion; aging