Western Blot (WB)
(CTDSPL Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CTDSPL antibody detected the CTDSPL protein (arrow).)
Rabbit anti-Human CTDSPL Polyclonal Antibody | anti-CTDSPL antibody
CTDSPL, NT (CTDSPL, C3orf8, NIF1, NIFL, SCP3, YA22, CTD small phosphatase-like protein, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, NIF-like protein, Nuclear LIM interactor-interacting factor 1, Protein YA22, RBSP3, Small
Gene Names
CTDSPL; PSR1; SCP3; HYA22; RBSP3; C3orf8
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CTDSPL; Polyclonal Antibody; NT (CTDSPL; C3orf8; NIF1; NIFL; SCP3; YA22; CTD small phosphatase-like protein; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; NIF-like protein; Nuclear LIM interactor-interacting factor 1; Protein YA22; RBSP3; Small; Anti -CTDSPL; anti-CTDSPL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-CTDSPL antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
CTDSPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human CTDSPL.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(CTDSPL Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CTDSPL antibody detected the CTDSPL protein (arrow).)
Western Blot (WB)
(CTDSPL Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CTDSPL antibody detected the CTDSPL protein (arrow).)
Flow Cytometry (FC/FACS)
(CTDSPL Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CTDSPL Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CTDSPL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,129 Da
NCBI Official Full Name
CTD small phosphatase-like protein isoform 1
NCBI Official Synonym Full Names
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like
NCBI Official Symbol
CTDSPL
NCBI Official Synonym Symbols
PSR1; SCP3; HYA22; RBSP3; C3orf8
NCBI Protein Information
CTD small phosphatase-like protein; CTDSP-like; NIF-like protein; small CTD phosphatase 3; NLI-interacting factor 1; small C-terminal domain phosphatase 3; nuclear LIM interactor-interacting factor 1; RB protein serine phosphatase from chromosome 3; carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3
UniProt Protein Name
CTD small phosphatase-like protein
Protein Family
UniProt Gene Name
CTDSPL
UniProt Synonym Gene Names
C3orf8; NIF1; NIFL; SCP3; YA22; CTDSP-like; NLI-interacting factor 1; hYA22; SCP3
UniProt Entry Name
CTDSL_HUMAN
Uniprot Description
CTDSPL: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.16; Phosphatase
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: nucleus
Molecular Function: metal ion binding; phosphoprotein phosphatase activity
Biological Process: dephosphorylation; negative regulation of protein amino acid phosphorylation
Research Articles on CTDSPL
Similar Products
Product Notes
The CTDSPL ctdspl (Catalog #AAA6001548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CTDSPL, NT (CTDSPL, C3orf8, NIF1, NIFL, SCP3, YA22, CTD small phosphatase-like protein, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, NIF-like protein, Nuclear LIM interactor-interacting factor 1, Protein YA22, RBSP3, Small reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTDSPL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CTDSPL ctdspl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTDSPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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