Goat CTDSPL Polyclonal Antibody | anti-CTDSPL antibody

Goat anti-CTDSPL Antibody

Cat. Num. AAA421907

• Gene Names: CTDSPL; PSR1; SCP3; HYA22; RBSP3; C3orf8
• Reactivity: Expected from sequence similarity: Human, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CTDSPL; Polyclonal Antibody; Goat anti-CTDSPL Antibody; CTD (carboxy-terminal domain; RNA polymerase II; polypeptide A) small phosphatase-like; C3orf8; HYA22; PSR1; RBSP3; SCP3; CTD small phosphatase-like protein; CTDSP-like; NIF-like protein; NLI-interacting factor 1; RB protein serine phosphatase fro; CTDSPL antibody; polypeptide A) small phosphatase-like antibody; C3orf8 antibody; HYA22 antibody; PSR1 antibody; SCP3 antibody; small CTD phosphatase 3 antibody; anti-CTDSPL antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QCNVSLKKQRSRS
• Sequence Length: 276

• Applicable Applications for anti-CTDSPL antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 20kDa band in human placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.1kDa according to NP_NP_001008393.1 and of 29.9kDa according to NP_005799.2. The 20kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1; NP_005799.2.
• Epitope: Internal region
• Note: This antibody is expected to recognise both isoforms (NP_001008393.1; NP_005799.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 56549683
• NCBI GeneID: 10217
• NCBI Accession #: NP_001008393.1; NP_005799.2
• NCBI GenBank Nucleotide #: NM_001008392.1
• Molecular Weight: 29,937 Da
• NCBI Official Full Name: CTD small phosphatase-like protein isoform 1
• NCBI Official Synonym Full Names: CTD small phosphatase like
• NCBI Official Symbol: CTDSPL
• NCBI Official Synonym Symbols: PSR1; SCP3; HYA22; RBSP3; C3orf8
• NCBI Protein Information: CTD small phosphatase-like protein
• UniProt Protein Name: CTD small phosphatase-like protein
• Protein Family: CTD small phosphatase-like protein
• UniProt Gene Name: CTDSPL
• UniProt Synonym Gene Names: C3orf8; NIF1; NIFL; SCP3; YA22; CTDSP-like; NLI-interacting factor 1; hYA22; SCP3; Small CTD phosphatase 3
• UniProt Entry Name: CTDSL_HUMAN

Uniprot Description

CTDSPL: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: nucleus

Molecular Function: metal ion binding; phosphoprotein phosphatase activity

Biological Process: negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation