Rabbit anti-Human CSNK2A3 Polyclonal Antibody | anti-CSNK2A3 antibody

CSNK2A3 Antibody

Cat. Num. AAA7113502

• Gene Names: CSNK2A3; CSNK2A1P
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%; Protein G Purified
• Synonyms: CSNK2A3; Polyclonal Antibody; CSNK2A3 Antibody; Casein kinase II subunit alpha 3; CK II alpha 3; EC 2.7.11.1; Casein kinase II alpha 1 polypeptide pseudogene; CSNK2A1P; anti-CSNK2A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 391

• Applicable Applications for anti-CSNK2A3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:200-1:500
• Immunogen Species: Human
• Immunogen: Recombinant Human Casein kinase II subunit alpha 3 protein (151-391AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysateAll lanes: CSNK2A3 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46 kDaObserved band size: 46 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

NCBI and Uniprot Product Information

• NCBI GI #: 375493534
• NCBI GeneID: 283106
• NCBI Accession #: NP_001243615.1
• NCBI GenBank Nucleotide #: NM_001256686.1
• UniProt Accession #: Q8NEV1
• Molecular Weight: 45,220 Da
• NCBI Official Full Name: casein kinase II subunit alpha 3
• NCBI Official Synonym Full Names: casein kinase 2 alpha 3
• NCBI Official Symbol: CSNK2A3
• NCBI Official Synonym Symbols: CSNK2A1P
• NCBI Protein Information: casein kinase II subunit alpha 3
• UniProt Protein Name: Casein kinase II subunit alpha 3
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK2A3
• UniProt Synonym Gene Names: CSNK2A1P; CK II alpha 3

NCBI Description

This gene encodes a protein that is highly similar to the casein kinase II alpha protein. Casein kinase II is a serine/threonine protein kinase complex that phosphorylates numerous substrates including casein. The alpha subunit is the catalytic component of the complex. Mutations in this gene may be associated with a susceptibility to lung cancer. There are contradictory views among published reports of this gene as to whether or not it is a protein-coding gene or a processed pseudogene (PMIDs: 20625391, 20625391 and 10094393). [provided by RefSeq, Feb 2012]

Uniprot Description

Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.