Rabbit anti-Human CSNK1E Polyclonal Antibody | anti-CSNK1E antibody

CSNK1E, CT (Casein Kinase I, epsilon Isoform, CKI-epsilon, CKIe) (FITC)

Cat. Num. AAA6467353

• Gene Names: CSNK1E; HCKIE; CKIepsilon
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CSNK1E; Polyclonal Antibody; CT (Casein Kinase I; epsilon Isoform; CKI-epsilon; CKIe) (FITC); anti-CSNK1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CK1e.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CSNK1E antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CK1e (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CK1e C-term Pab in SKBR3 cell lysate. CK1e (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CSNK1E antibody

Antibodies; Protein Kinases

References

1. Hino, S., et al., J. Biol. Chem. 278(16):14066-14073 (2003). 2. Eide, E.J., et al., J. Biol. Chem. 277(19):17248-17254 (2002). 3. Keesler, G.A., et al., Neuroreport 11(5):951-955 (2000). 4. Cegielska, A., et al., J. Biol. Chem. 273(3):1357-1364 (1998). 5. Kloss, B., et al., Cell 94(1):97-107 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4503093
• NCBI GeneID: 1454
• NCBI Accession #: NP_001885.1
• NCBI GenBank Nucleotide #: NM_001894.4
• UniProt Accession #: P49674
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Synonym Full Names: casein kinase 1, epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; CKIepsilon
• NCBI Protein Information: casein kinase I isoform epsilon; CKIe; CKI-epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; regulation of circadian rhythm; DNA repair; signal transduction; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle