Rabbit anti-Human CSK Polyclonal Antibody | anti-CSK antibody

CSK, NT (CSK, Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL) (APC)

Cat. Num. AAA6289309

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSK; Polyclonal Antibody; NT (CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL) (APC); Protein-tyrosine kinase CYL; anti-CSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CSK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human CSK.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CSK Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CSK antibody detected the CSK protein (arrow).)

Related Product Information for anti-CSK antibody

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

Product Categories/Family for anti-CSK antibody

Antibodies; Protein Kinases

References

Niu, W., et al. Clin. Chim. Acta 411 (19-20), 1491-1495 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Mikkola, E.T., et al. J. Mol. Biol. 399(4):618-627(2010). Takeuchi, F., et al. Circulation 121(21):2302-2309(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1; NP_004374.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK; C-Src kinase; protein-tyrosine kinase CYL
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; negative regulation of kinase activity; negative regulation of phagocytosis; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cytokine production; regulation of cell proliferation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; T cell costimulation; negative regulation of interleukin-6 production; innate immune response; oligodendrocyte differentiation; morphogenesis of an epithelium; brain development; negative regulation of bone resorption; cell differentiation; blood coagulation; transmembrane receptor protein tyrosine kinase signaling pathway