anti-Drosophila Cryptochrome Polyclonal Antibody | anti-phrA antibody

Anti - Cryptochrome (CRY)

Cat. Num. AAA611649

• Reactivity: Drosophila
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Cryptochrome; Polyclonal Antibody; Anti - Cryptochrome (CRY); Cryptochrome (CRY); anti-phrA antibody

• Host: Host: Rabbit; Source: Drosophila
• Reactivity: Drosophila
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The mouse peptide sequence is 92% conserved in flesh fly and 76% in Chinese silk moth cryptochrome. No significant homology is seen with mammalian CRY1/2. Antibody crossreactivity with CRY from various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
• Sequence Length: 500

• Applicable Applications for anti-phrA antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.; Optimal dilutions to be determined by the researcher.
• Immunogen: An 18 aa peptide sequence within the C-terminus of Drosophila CRY (1) was synthesized coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-phrA antibody

Circadian rhythm is one of the most fascinating and complex biological phenomenon\'s. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules.

Product Categories/Family for anti-phrA antibody

Abs to Receptors

References

• Emery P et al (1998) Cell 95, 669-679; Stanewsky R et al (1998) Cell 95, 681-692; Miyamoto Y and Sancar A (1998) 95, 6097-6102

NCBI and Uniprot Product Information

• NCBI GI #: 37520404
• NCBI GeneID: 2599355
• NCBI Accession #: NP_923781.1
• NCBI GenBank Nucleotide #: NC_005125.1
• NCBI Official Full Name: cryptochrome
• NCBI Official Symbol: phrA
• NCBI Protein Information: cryptochrome
• Protein Family: Cryptochrome