Immunohistochemistry (IHC)
(Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) using MBS6000709.)
Rabbit CRYAB Polyclonal Antibody | anti-CRYAB antibody
CRYAB, CT (Alpha-crystallin B Chain, Alpha(B)-crystallin, Heat Shock Protein beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27, Rosenthal Fiber Component, CRYA2)
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CRYAB; Polyclonal Antibody; CT (Alpha-crystallin B Chain; Alpha(B)-crystallin; Heat Shock Protein beta-5; HspB5; Renal Carcinoma Antigen NY-REN-27; Rosenthal Fiber Component; CRYA2); Anti -CRYAB; anti-CRYAB antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CRYAB. Species Crossreactivity: bovine, mouse, porcine and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Applicable Applications for anti-CRYAB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (FFPE): 10ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with C7944-60E, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Dilution: Western Blot: 1:1000
Immunohistochemistry (FFPE): 10ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with C7944-60E, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Immunogen
Synthetic peptide corresponding to C-terminus of human alphaB-Crystallin conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) using MBS6000709.)
Immunohistochemistry (IHC)
(Brain, cortex, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) using MBS6000709.)
Immunohistochemistry (IHC)
(Heart, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) using MBS6000709.)
Immunohistochemistry (IHC)
(Heart, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) using MBS6000709.)
Western Blot (WB)
(Western Blot Analysis of SPA-223: Lane 1: MWM, Lane 2: SPP-225 Lane3: SPP-226 , Lane4: SPP-227, Lane 5: SPP-235)
Western Blot (WB)
(Western Blot Analysis of SPA-223: Lane 1: MWM, Lane 2: SPP-225 Lane3: SPP-226 , Lane4: SPP-227, Lane 5: SPP-235)
Related Product Information for anti-CRYAB antibody
Crystallin AB (CryAB) is a developmentally regulated small heat shock protein known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is known to be associated with a number of neurodegenerative pathologies such as Alzheimer disease amd multiple sclerosis. As well, it has been found to be expressed in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.
Product Categories/Family for anti-CRYAB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20,129 Da
NCBI Official Full Name
CRYAB
NCBI Official Symbol
CRYAB
NCBI Protein Information
crystallin, alpha B; Alpha(B)-crystallin; Alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
Protein Family
UniProt Gene Name
CRYAB
UniProt Entry Name
CRYAB_PIG
Uniprot Description
CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Protein type: Chaperone; Heat shock protein
Cellular Component: mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus; Z disc
Molecular Function: protein homodimerization activity; metal ion binding; unfolded protein binding; structural constituent of eye lens
Biological Process: tubulin folding; lens development in camera-type eye; muscle development; negative regulation of intracellular transport; response to hydrogen peroxide; response to hypoxia; negative regulation of caspase activity; protein homooligomerization
Similar Products
Product Notes
The CRYAB cryab (Catalog #AAA6000709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRYAB, CT (Alpha-crystallin B Chain, Alpha(B)-crystallin, Heat Shock Protein beta-5, HspB5, Renal Carcinoma Antigen NY-REN-27, Rosenthal Fiber Component, CRYA2) reacts with Bovine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CRYAB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:1000 Immunohistochemistry (FFPE): 10ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with C7944-60E, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Researchers should empirically determine the suitability of the CRYAB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRYAB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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