Rabbit anti-Human, Mouse CRSP77 Polyclonal Antibody | anti-CRSP77 antibody

CRSP77 (V171) Polyclonal Antibody

Cat. Num. AAA3002510

• Gene Names: MED17; SRB4; CRSP6; CRSP77; DRIP80; TRAP80
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CRSP77; Polyclonal Antibody; CRSP77 (V171) Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 17; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; Mediator complex subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Trap80; Transcriptional coactivator CRSP77; Vitamin D3 receptor-interacting protein complex 80 kDa component; DRIP80; MED17; CRSP6; DRIP77; TRAP80; anti-CRSP77 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CRSP77 (V171) polyclonal antibody detects endogenous levels of CRSP77 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 651

• Applicable Applications for anti-CRSP77 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 140-190 of Human CRSP77.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CRSP77 (V171) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-CRSP77 antibody

CRSP is ubiquitously expressed in various tissues and functions as a multimeric complex that consists of nine distinct subunits, each having an individual molecular mass ranging from 33 to 200 kDa. Several members of the CRSP family share sequence similarity with multiple components of the yeast transcriptional mediator proteins, including CRSP150, which is related to yeast Rgr1, and CRSP70, which is similar to the elongation factor TFIIS. CRSP77 and CRSP150 are also related to proteins within the putative murine mediator complex, while CRSP130 and CRSP34 are largely unrelated to either murine or yeast proteins. CRSP subunits also associate with larger multimeric co-activator complexes, including ARC/DRI, which binds directly to SREBP and nuclear hormone receptors to facilitate transcription, and with NAT, a polymerase II-interacting complex

NCBI and Uniprot Product Information

• NCBI GI #: 28558975
• NCBI GeneID: 9440
• NCBI Accession #: NP_004259.3
• NCBI GenBank Nucleotide #: NP_004259.3
• UniProt Accession #: Q9NVC6
• Molecular Weight: ~ 73kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 17
• NCBI Official Synonym Full Names: mediator complex subunit 17
• NCBI Official Symbol: MED17
• NCBI Official Synonym Symbols: SRB4; CRSP6; CRSP77; DRIP80; TRAP80
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 17
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 17
• UniProt Gene Name: MED17
• UniProt Synonym Gene Names: ARC77; CRSP6; DRIP77; DRIP80; TRAP80; ARC77; CRSP complex subunit 6; Trap80; DRIP80
• UniProt Entry Name: MED17_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]

Uniprot Description

MED17: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Defects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA). It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth. Belongs to the Mediator complex subunit 17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: nucleoplasm; transcription factor complex; membrane; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; stem cell maintenance; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter

Disease: Microcephaly, Postnatal Progressive, With Seizures And Brain Atrophy