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Western Blot (WB) (Western blotAll lanes: CROT antibody at 3ug/mlLane 1:Mouse kidney tissueLane 2:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 71,11,74 kDaObserved band size: 70 kDa)

Rabbit anti-Human, Mouse CROT Polyclonal Antibody | anti-CROT antibody

CROT Antibody

Gene Names
CROT; COT
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
CROT; Polyclonal Antibody; CROT Antibody; COT; anti-CROT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
640
Applicable Applications for anti-CROT antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Peroxisomal carnitine O-octanoyltransferase protein (1-87aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-162717 / sc-162715
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: CROT antibody at 3ug/mlLane 1:Mouse kidney tissueLane 2:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 71,11,74 kDaObserved band size: 70 kDa)

Western Blot (WB) (Western blotAll lanes: CROT antibody at 3ug/mlLane 1:Mouse kidney tissueLane 2:A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 71,11,74 kDaObserved band size: 70 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer gland using MBS1494373 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer gland using MBS1494373 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissuegland using MBS1494373 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissuegland using MBS1494373 at dilution 1:100)
Related Product Information for anti-CROT antibody
Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,383 Da
NCBI Official Full Name
peroxisomal carnitine O-octanoyltransferase isoform 1
NCBI Official Synonym Full Names
carnitine O-octanoyltransferase
NCBI Official Symbol
CROT
NCBI Official Synonym Symbols
COT
NCBI Protein Information
peroxisomal carnitine O-octanoyltransferase
UniProt Protein Name
Peroxisomal carnitine O-octanoyltransferase
Protein Family
UniProt Gene Name
CROT
UniProt Synonym Gene Names
COT; COT
UniProt Entry Name
OCTC_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Uniprot Description

CROT: Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester. Belongs to the carnitine/choline acetyltransferase family.

Protein type: Transferase; EC 2.3.1.137

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intracellular membrane-bound organelle; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: carnitine O-octanoyltransferase activity; receptor binding

Biological Process: carnitine metabolic process; cellular lipid metabolic process; coenzyme A metabolic process; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA oxidase; fatty acid metabolic process; fatty acid transport; generation of precursor metabolites and energy; medium-chain fatty acid metabolic process; response to drug; response to organic nitrogen

Research Articles on CROT

Similar Products

Product Notes

The CROT crot (Catalog #AAA1494373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CROT Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CROT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the CROT crot for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CROT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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