Rabbit anti-Human CRK Polyclonal Antibody | anti-CRK antibody

CRK, Phosphorylated (Ser41) (Adapter Molecule crk, Proto-oncogene c-Crk, p38) (FITC)

Cat. Num. AAA6470681

• Gene Names: CRK; p38; CRKII
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRK; Polyclonal Antibody; Phosphorylated (Ser41) (Adapter Molecule crk; Proto-oncogene c-Crk; p38) (FITC); anti-CRK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRK when phosphorylated at Ser41.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CRK antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser41 of human CRK (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-CRK-S41 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Product Categories/Family for anti-CRK antibody

Antibodies; Disease Markers

References

1. Bougneres, L., et al., J. Cell Biol. 166(2):225-235 (2004). 2. Stoletov, K.V., et al., Exp. Cell Res. 295(1):258-268 (2004). 3. Miller, C.T., et al., Oncogene 22(39):7950-7957 (2003). 4. Sun, J., et al., J. Biol. Chem. 278(35):32794-32800 (2003). 5. Zhang, X.A., et al., J. Biol. Chem. 278(29):27319-27328 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 41327710
• NCBI GeneID: 1398
• NCBI Accession #: NP_005197
• NCBI GenBank Nucleotide #: NM_005206.4
• UniProt Accession #: Q96GA9; Q96HJ0; A8MWE8; B0LPE8; D3DTH6; P46108
• Molecular Weight: 25.0 kDa (224aa), confirmed by MALDI-TOF
• NCBI Official Full Name: adapter molecule crk isoform b
• NCBI Official Synonym Full Names: CRK proto-oncogene, adaptor protein
• NCBI Official Symbol: CRK
• NCBI Official Synonym Symbols: p38; CRKII
• NCBI Protein Information: adapter molecule crk
• Protein Family: Crk-like protein

NCBI Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]