Rabbit anti-Human CRIPTO Polyclonal Antibody | anti-CRIPTO antibody

CRIPTO, NT (Teratocarcinoma-derived Growth Factor 1, Epidermal Growth Factor-like Cripto Protein CR1, Cripto-1 Growth Factor, CRGF, TDGF1) (APC)

Cat. Num. AAA6470635

• Gene Names: TDGF1; CR; CRGF; CRIPTO
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CRIPTO; Polyclonal Antibody; NT (Teratocarcinoma-derived Growth Factor 1; Epidermal Growth Factor-like Cripto Protein CR1; Cripto-1 Growth Factor; CRGF; TDGF1) (APC); anti-CRIPTO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRIPTO.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CRIPTO antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CRIPTO (TDGF1) Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TDGF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CRIPTO antibody

Antibodies; Growth Factors; Cytokines

References

1. Parisi, S., et al., J. Cell Biol. 163(2):303-314 (2003). 2. Gray, P.C., et al., Proc. Natl. Acad. Sci. USA 100(9):5193-5198 (2003). 3. Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002). 4. de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002). 5. Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4507425
• NCBI GeneID: 6997
• NCBI Accession #: NP_003203
• NCBI GenBank Nucleotide #: NM_003212.3
• UniProt Accession #: P13385; Q8TCC1
• Molecular Weight: 21 kDa
• NCBI Official Full Name: teratocarcinoma-derived growth factor 1 isoform 1
• NCBI Official Synonym Full Names: teratocarcinoma-derived growth factor 1
• NCBI Official Symbol: TDGF1
• NCBI Official Synonym Symbols: CR; CRGF; CRIPTO
• NCBI Protein Information: teratocarcinoma-derived growth factor 1; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1
• UniProt Protein Name: Teratocarcinoma-derived growth factor 1
• UniProt Gene Name: TDGF1
• UniProt Synonym Gene Names: CRIPTO; CRGF
• UniProt Entry Name: TDGF1_HUMAN

NCBI Description

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Cell cycle regulation; Membrane protein, peripheral; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; growth factor activity; receptor binding

Biological Process: activation of MAPK activity; somatic stem cell maintenance; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of fibroblast proliferation; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; cell differentiation; vasculogenesis; positive regulation of cell-matrix adhesion; epidermal growth factor receptor signaling pathway; in utero embryonic development; morphogenesis of a branching structure; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; peptidyl-serine phosphorylation; cell migration during sprouting angiogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; negative regulation of apoptosis