Goat CREB3L2 Polyclonal Antibody | anti-CREB3L2 antibody

Goat anti-CREB3L2 Antibody

Cat. Num. AAA423185

• Gene Names: CREB3L2; BBF2H7
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CREB3L2; Polyclonal Antibody; Goat anti-CREB3L2 Antibody; Goat Anti-CREB3L2 / BBF2H7 Antibody; cAMP responsive element binding protein 3-like 2; BBF2H7; B-ZIB transcription factor; BBF2 human homolog on chromosome 7; FUS/BBF2H7 protein; TCAG_1951439; basic transcription factor 2; cAMP-responsive element-binding protein 3-like protein 2; cy; CREB3L2 antibody; cAMP responsive element binding protein 3-like 2 antibody; BBF2H7 antibody; B-ZIB transcription factor antibody; BBF2 human homolog on chromosome 7 antibody; FUS/BBF2H7 protein antibody; TCAG_1951439 antibody; basic transcription factor 2 antibody; cAMP-responsive element-binding protein 3-like protein 2 antibody; cyclic AMP-responsive element-binding protein 3-like protein 2 antibody; anti-CREB3L2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DRKLSELSEPGDGE
• Sequence Length: 520

• Applicable Applications for anti-CREB3L2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Placenta shows staining in select nuclei. Recommended concentration, 5-10ug/ml.; Western Blot: Approx 26kDa band observed in Human Placenta lysates (calculated MW of 27.7kDa according to NP_001240704.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence DRKLSELSEPGDGE-C, from the N Terminus of the protein sequence according to NP_919047.2; NP_001240704.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognize both reported isoforms (NP_919047.2; NP_001240704.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 56550067
• NCBI GeneID: 64764
• NCBI Accession #: NP_919047.2; NP_001240704.1
• NCBI GenBank Nucleotide #: NM_194071.3
• Molecular Weight: 27,722 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 3-like protein 2 isoform 1
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3-like 2
• NCBI Official Symbol: CREB3L2
• NCBI Official Synonym Symbols: BBF2H7
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3-like protein 2
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3-like protein 2
• Protein Family: Cyclic AMP-responsive element-binding protein 3-like protein
• UniProt Gene Name: CREB3L2
• UniProt Synonym Gene Names: BBF2H7; cAMP-responsive element-binding protein 3-like protein 2
• UniProt Entry Name: CR3L2_HUMAN

NCBI Description

This gene encodes a member of the oasis bZIP transcription factor family. Members of this family can dimerize but form homodimers only. The encoded protein is a transcriptional activator. Translocations between this gene on chromosome 7 and the gene fused in sarcoma on chromosome 16 can be found in some tumors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CREB3L2: Transcriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage. A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS. Belongs to the bZIP family. ATF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; nucleoplasm; nucleus

Biological Process: cartilage development; chondrocyte differentiation; ER to Golgi vesicle-mediated transport; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; unfolded protein response