Rabbit anti-Human CREB Polyclonal Antibody | anti-CREB antibody

CREB (cAMP Response Element Binding Protein) (APC)

Cat. Num. AAA6470624

• Gene Names: CREB1; CREB; CREB-1
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CREB; Polyclonal Antibody; CREB (cAMP Response Element Binding Protein) (APC); anti-CREB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CREB at 43kD. Species crossreactivity: Broad species crossreactivity expected due to conservation of immunogenic sequence.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CREB antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 5mg (will immunoprecipitated CREB from 500mg of HeLa nuclear extract).; WB: 0.5-2mg/ml detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.
• Conjugate: APC
• Positive Control: HeLa nuclear extract. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CREB antibody

Antibodies; Transcription Factors

References

Jean, D., et al., J. Biol. Chem. 273: 24884-24890, 1998. Lundblad, J., et al., J. Biol. Chem. 273: 19251-19259, 1998. Radhakrishnan, I., et al., FEBS Lett. 430: 317-322, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 19745184
• NCBI GeneID: 1385
• NCBI Accession #: NP_604391
• NCBI GenBank Nucleotide #: NM_134442
• UniProt Accession #: P16220
• Molecular Weight: 37kDa
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB; CREB-1
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: CREB-binding protein
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; lactation; axon guidance; response to glucagon stimulus; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; response to organic substance; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; secretory granule organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; phospholipase C activation; pituitary gland development; toll-like receptor signaling pathway; positive regulation of fat cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size

Disease: Histiocytoma, Angiomatoid Fibrous