Rabbit anti-Human CRABP2 Polyclonal Antibody | anti-CRABP2 antibody

CRABP2, CT (CRABP2, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II)

Cat. Num. AAA647647

• Gene Names: CRABP2; RBP6; CRABP-II
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CRABP2; Polyclonal Antibody; CT (CRABP2; Cellular retinoic acid-binding protein 2; Cellular retinoic acid-binding protein II); Anti -CRABP2; anti-CRABP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CRABP2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: CRABP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-116 amino acids from the C-terminal region of human CRABP2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CRABP2 Antibody (C-term) western blot analysis MDA-MB453 cell line lysates (35ug/lane).This demonstrates the CRABP2 antibody detected the CRABP2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of CRABP2 (arrow) using rabbit polyclonal CRABP2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRABP2 gene.)

Related Product Information for anti-CRABP2 antibody

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. [provided by RefSeq].

Product Categories/Family for anti-CRABP2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 315013542
• NCBI GeneID: 1382
• NCBI Accession #: NP_001186652.1
• NCBI GenBank Nucleotide #: NM_001199723.1
• UniProt Accession #: P29373; Q6ICN6; B2R4Z8; D3DVC5; F1T098
• Molecular Weight: 15,693 Da
• NCBI Official Full Name: cellular retinoic acid-binding protein 2
• NCBI Official Synonym Full Names: cellular retinoic acid binding protein 2
• NCBI Official Symbol: CRABP2
• NCBI Official Synonym Symbols: RBP6; CRABP-II
• NCBI Protein Information: cellular retinoic acid-binding protein 2; cellular retinoic acid-binding protein II
• UniProt Protein Name: Cellular retinoic acid-binding protein 2
• Protein Family: Cellular retinoic acid-binding protein
• UniProt Gene Name: CRABP2
• UniProt Synonym Gene Names: CRABP-II
• UniProt Entry Name: RABP2_HUMAN

NCBI Description

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

Function: Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

Subunit structure: Interacts with RXR and RARA

By similarity. Interacts with importin alpha. Ref.11

Subcellular location: Cytoplasm. Endoplasmic reticulum. Nucleus. Note: Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Ref.11 Ref.12

Induction: By retinoic acid.

Domain: Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Post-translational modification: Sumoylated in response to retinoic acid binding, sumoylation is critical for dissociation from ER and subsequent nuclear translocation. Ref.14

Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.