Rabbit anti-Human CPT1B Polyclonal Antibody | anti-CPT1B antibody

CPT1B, CT (Carnitine O-palmitoyltransferase 1, Muscle Isoform, CPT1-M, EC 2.3.1.21, Carnitine O-palmitoyltransferase I, Muscle Isoform, CPTI-M, CPT I, Carnitine Palmitoyltransferase 1B, Carnitine Palmitoyltransferase I-like Protein, CPT1B, KIAA1670) (FITC

Cat. Num. AAA6470432

• Gene Names: CPT1B; CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CPT1B; Polyclonal Antibody; CT (Carnitine O-palmitoyltransferase 1; Muscle Isoform; CPT1-M; EC 2.3.1.21; Carnitine O-palmitoyltransferase I; CPTI-M; CPT I; Carnitine Palmitoyltransferase 1B; Carnitine Palmitoyltransferase I-like Protein; KIAA1670) (FITC; anti-CPT1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPT1B.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CPT1B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CPT1B (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPT1B (arrow) using rabbit polyclonal CPT1B Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CPT1B antibody

Antibodies; Proteins

References

1. Dai, J., et al., Biochem. Biophys. Res. Commun. 301(3):758-763 (2003). 2. van der Leij, F.R., et al., J. Biol. Chem. 277(30):26994-27005 (2002). 3. Hirosawa, M., et al., DNA Res. 8(1):1-9 (2001). 4. Yamazaki, N., et al., J. Biol. Chem. 275(41):31739-31746 (2000). 5. Britton, C.H., et al., Genomics 40(1):209-211 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4758050
• NCBI GeneID: 1375
• NCBI Accession #: NP_004368
• NCBI GenBank Nucleotide #: NM_004377
• UniProt Accession #: Q92523
• Molecular Weight: 88kDa
• NCBI Official Full Name: carnitine O-palmitoyltransferase 1, muscle isoform isoform a
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1B
• NCBI Official Symbol: CPT1B
• NCBI Official Synonym Symbols: CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, muscle isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, muscle isoform
• Protein Family: Carnitine O-palmitoyltransferase
• UniProt Gene Name: CPT1B
• UniProt Synonym Gene Names: KIAA1670; CPT1-M; CPT I; CPTI-M
• UniProt Entry Name: CPT1B_HUMAN

NCBI Description

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

CPT1B: a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Protein type: Lipid Metabolism - fatty acid; Membrane protein, multi-pass; Membrane protein, integral; Transferase; EC 2.3.1.21

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: carnitine O-palmitoyltransferase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process; carnitine shuttle