Rabbit anti-Human CPN1 Polyclonal Antibody | anti-CPN1 antibody

CPN1, ID (CPN1, ACBP, Carboxypeptidase N catalytic chain, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carbo

Cat. Num. AAA6288313

• Gene Names: CPN1; CPN; SCPN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CPN1; Polyclonal Antibody; ID (CPN1; ACBP; Carboxypeptidase N catalytic chain; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carbo; Serum carboxypeptidase N; anti-CPN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-CPN1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-240 amino acids from the Central region of human CPN1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.)

Immunohistochemistry (IHC)

(CPN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CPN1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CPN1 antibody

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq].

Product Categories/Family for anti-CPN1 antibody

Antibodies; Enzymes; Carboxypeptidase

References

Speliotes, E.K., et al. Hepatology 52(3):904-912(2010). Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010). Du, X.Y., et al. J. Biol. Chem. 284(2):751-758(2009). Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008). Skidgel, R.A., et al. Int. Immunopharmacol. 7(14):1888-1899(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503011
• NCBI GeneID: 1369
• NCBI Accession #: NP_001299.1
• NCBI GenBank Nucleotide #: NM_001308.2
• UniProt Accession #: P15169
• Molecular Weight: 52,286 Da
• NCBI Official Full Name: carboxypeptidase N catalytic chain
• NCBI Official Synonym Full Names: carboxypeptidase N, polypeptide 1
• NCBI Official Symbol: CPN1
• NCBI Official Synonym Symbols: CPN; SCPN
• NCBI Protein Information: carboxypeptidase N catalytic chain; kininase I; kininase-1; carboxypeptidase K; lysine carboxypeptidase; serum carboxypeptidase N; anaphylatoxin inactivator; arginine carboxypeptidase; plasma carboxypeptidase B; carboxypeptidase N small subunit; carboxype
• UniProt Protein Name: Carboxypeptidase N catalytic chain
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN1
• UniProt Synonym Gene Names: ACBP; CPN; SCPN
• UniProt Entry Name: CBPN_HUMAN

NCBI Description

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.3; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency