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Western Blot (WB) (Gel: 10+15%SDS-PAGE, Lysate: 50 ug, Lane: 293T cells, Primary antibody: 820115(COX7B Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human COX7B Polyclonal Antibody | anti-COX7B antibody

COX7B Rabbit pAb

Gene Names
COX7B; APLCC; LSDMCA2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
COX7B; Polyclonal Antibody; COX7B Rabbit pAb; Cytochrome c oxidase subunit VIIb; anti-COX7B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-COX7B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Fusion protein of human COX7B
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Membrane, Mitochondrion, Mitochondrion inner membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 10+15%SDS-PAGE, Lysate: 50 ug, Lane: 293T cells, Primary antibody: 820115(COX7B Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 10+15%SDS-PAGE, Lysate: 50 ug, Lane: 293T cells, Primary antibody: 820115(COX7B Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 820115(COX7B Antibody) at dilution 1/60. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 820115(COX7B Antibody) at dilution 1/60. (Original magnification: ×200))
Product Categories/Family for anti-COX7B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
9,161 Da
NCBI Official Full Name
Homo sapiens cytochrome c oxidase subunit VIIb, mRNA
NCBI Official Synonym Full Names
cytochrome c oxidase subunit 7B
NCBI Official Symbol
COX7B
NCBI Official Synonym Symbols
APLCC; LSDMCA2
NCBI Protein Information
cytochrome c oxidase subunit 7B, mitochondrial
UniProt Protein Name
Cytochrome c oxidase subunit 7B, mitochondrial
Protein Family
UniProt Gene Name
COX7B
UniProt Entry Name
COX7B_HUMAN

NCBI Description

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes subunit VIIb, which is highly similar to bovine COX VIIb protein and is found in all tissues. This gene may have several pseudogenes on chromosomes 1, 2, 20 and 22. [provided by RefSeq, Jun 2011]

Uniprot Description

COX7B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase VIIb family.

Protein type: Membrane protein, integral; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain

Molecular Function: cytochrome-c oxidase activity

Biological Process: cellular metabolic process; central nervous system development; transmembrane transport

Disease: Aplasia Cutis Congenita, Reticulolinear, With Microcephaly, Facial Dysmorphism, And Other Congenital Anomalies

Research Articles on COX7B

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Product Notes

The COX7B cox7b (Catalog #AAA4757739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COX7B Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COX7B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the COX7B cox7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX7B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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