Rabbit COVID 19 Nucleocapsid (NP) Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

Rabbit anti SARS-CoV2 (N) polyclonal antibody

Cat. Num. AAA462259

• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
• Purity: The Rabbit IgG is purified by site-directed Epitope Affinity Purification
• Synonyms: COVID 19 Nucleocapsid (NP) Coronavirus; Polyclonal Antibody; Rabbit anti SARS-CoV2 (N) polyclonal antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); SARS-CoV-2(N) nucleocapsid protein; anti-COVID-19 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This antibody recognizes SARS-Cov2 Nucleocapsid protein.
• Purity/Purification: The Rabbit IgG is purified by site-directed Epitope Affinity Purification
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC)
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; IHC: 1:100 ug/ml; Flow cytometry: Not tested; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preperation: A synthetic corresponding to N-terminus of SARS-COV2-Neucleocapsid Protein.
• Positive Control: Sars-Cov2 infected lung tissue
• Cellular Location: Cytoplasma
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.; The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity.; Avoid repeated freezing-thawing cycles.

Immunohistochemistry (IHC)

(IHC: Human Lung Tissue stained with Rabbit anti SARS-Cov2 (N) antibody, MBS462259 at 1:100 for 10 minutes at room tempeture.Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH6.0 for 10 minutes followed by cooling at room tempeture for 20 minutes.Fig 1 Positive alveolar macrophages in the lung tissue;Figure 2 is a negative staining of Lung tissue.)

Related Product Information for anti-COVID-19 antibody

SARS-CoV-2, which causes the COVID-19 disease, is a novel coronavirus which results in an acute respiratory syndrome. It is an enveloped positive-sense RNA virus. SARS-CoV-2 contains four structural proteins: E (envelope), M (membrane), S (spike), and N (nucleocapsid) proteins. The N protein holds the RNA genome, and the S, E, and M proteins form the viral envelope. Coronavirus S protein is a large, multifunctional class I viral transmembrane protein. It is required for the entry of the virion particles into the cell through a contact with host cellular receptors . The S-protein of coronavirus consists of S1 and S2 functional units. S1 is responsible for host receptor binding, S2 is in charge of fusion . The nucleocapsid (N) is a 45 kDa protein, one of the major structural proteins of the virus. It binds directly to viral RNA and provides stability. N-protein is involved in the transcription and viral RNA replication, packaging of the genome into helical ribonucleocapsid , and interference with cell cycle processes of host cells.