SDS-Page
(Detection of Serpin A6 with MBS621456. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL goat anti-rSerpin A6.)
anti-Human Cortisol Binding Globulin Polyclonal Antibody
Anti - Cortisol Binding Globulin (CBG, Corticosteroid Binding Globulin, Serine (or Cysteine) Proteinase Inhibitor Clade A (alpha-1 Antiproteinase, Antitrypsin) Member 6, Serpin Peptidase Inhibitor Clade A (alpha-1 Antiproteinase, Antitrypsin) Member 6, Se
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
Cortisol Binding Globulin; Polyclonal Antibody; Anti - Cortisol Binding Globulin (CBG; Corticosteroid Binding Globulin; Serine (or Cysteine) Proteinase Inhibitor Clade A (alpha-1 Antiproteinase; Antitrypsin) Member 6; Serpin Peptidase Inhibitor Clade A (alpha-1 Antiproteinase; Se; Cortisol Binding Globulin (CBG; Serpin A6; anti-Cortisol Binding Globulin antibody
Host
Host: Goat
Source: Rat
Source: Rat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Serpin A6. Species Crossreactivity: < 5% cross-reactivity with rmSerpin A1, rmSerpin A5 and rhSerpin A4.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Affinity Purified
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Applicable Applications for anti-Cortisol Binding Globulin antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA and Western Blot. Other applications not tested.
Dilution: ELISA (direct): 0.5-1.0ug/ml
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Dilution: ELISA (direct): 0.5-1.0ug/ml
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Immunogen
NS0-derived rrSerpin A6
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile buffer. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Serpin A6 with MBS621456. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL goat anti-rSerpin A6.)
SDS-Page
(Detection of Serpin A6 with MBS621456. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL goat anti-rSerpin A6.)
Related Product Information for anti-Cortisol Binding Globulin antibody
Serpin A6 (also corticosteroid-binding globulin/CBG and Transcortin) is a 45-60 kDa, secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Mature rat Serpin A6 is 374 amino acids (aa) in length (aa 23-396). It
Product Categories/Family for anti-Cortisol Binding Globulin antibody
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Product Notes
The Cortisol Binding Globulin (Catalog #AAA621456) is an Antibody produced from Host: Goat Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - Cortisol Binding Globulin (CBG, Corticosteroid Binding Globulin, Serine (or Cysteine) Proteinase Inhibitor Clade A (alpha-1 Antiproteinase, Antitrypsin) Member 6, Serpin Peptidase Inhibitor Clade A (alpha-1 Antiproteinase, Antitrypsin) Member 6, Se reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cortisol Binding Globulin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA and Western Blot. Other applications not tested. Dilution: ELISA (direct): 0.5-1.0ug/ml Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Cortisol Binding Globulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortisol Binding Globulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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