Western Blot (WB)
(Western Blot analysis of LOVO+H2O2 cells using Phospho-Cortactin (Y421) Polyclonal Antibody)
Rabbit Cortactin Polyclonal Antibody | anti-CTTN antibody
Cortactin (Phospho-Tyr421) Polyclonal Antibody
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:20000
Not yet tested in other applications
Western Blot (WB)
(Western Blot analysis of LOVO+H2O2 cells using Phospho-Cortactin (Y421) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of LOVO+H2O2 cells using Phospho-Cortactin (Y421) Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
Uniprot Description
Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: cortical cytoskeleton; ruffle; voltage-gated potassium channel complex; focal adhesion; growth cone; cytoskeleton; lamellipodium; cytoplasm; dendritic spine; podosome; actin filament; coated pit; cell cortex
Molecular Function: protein binding
Biological Process: focal adhesion formation; receptor-mediated endocytosis; intracellular protein transport; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis
Research Articles on CTTN
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Product Notes
The CTTN cttn (Catalog #AAA9407572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cortactin (Phospho-Tyr421) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cortactin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the CTTN cttn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortactin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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