Rabbit anti-Human COPB1 Polyclonal Antibody | anti-COPB1 antibody

COPB1, ID (COPB1, COPB, Coatomer subunit beta, Beta-coat protein)

Cat. Num. AAA6003791

• Gene Names: COPB1; Beta-COP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: COPB1; Polyclonal Antibody; ID (COPB1; COPB; Coatomer subunit beta; Beta-coat protein); Anti -COPB1; anti-COPB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-COPB1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: COPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-380 amino acids from the Central region of human COPB1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COPB1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the COPB1 antibody detected the COPB1 protein (arrow).)

Related Product Information for anti-COPB1 antibody

This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-COPB1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 288860138
• NCBI GeneID: 100337660
• NCBI Accession #: NP_001165837.1
• NCBI GenBank Nucleotide #: NM_001172366.1
• UniProt Accession #: D2SW95
• Molecular Weight: 107,123 Da
• NCBI Official Full Name: coatomer subunit beta
• NCBI Official Symbol: COPB1
• NCBI Official Synonym Symbols: Beta-COP
• NCBI Protein Information: coatomer subunit beta; Beta-coat protein; coatomer protein subunit beta 1
• UniProt Protein Name: Coatomer subunit beta
• UniProt Gene Name: COPB1
• UniProt Synonym Gene Names: Beta-COP
• UniProt Entry Name: COPB_PIG

Uniprot Description

Function: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1

By similarity.

Subunit structure: Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits

By similarity. Interacts with CAPN8 and PRKCE

By similarity. Interacts with SCYL1

By similarity. Interacts with COPG1

By similarity. Interacts with ARF1 (myristoylated); this interaction is required for binding of COPB1 to Golgi membranes

By similarity. Interacts (via trunk domain) with ARF1 (via switch I region); the interaction is direct

By similarity. Interacts with KCNK2 (via N-terminus); this interaction increases the channel-mediated whole cell currents and promotes plasma membrane expression of KCNK2

By similarity. Interacts with STX17

By similarity.

Subcellular location: Cytoplasm

By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cell membrane

By similarity. Endoplasmic reticulum-Golgi intermediate compartment

By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it

By similarity. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm

By similarity. Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack

By similarity. Occassionally present at the trans- side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae

By similarity. Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles

By similarity. Some association with high-density and low-density microsomes and mitochondria/nuclei fraction

By similarity. Very little found in plasma membrane fraction

By similarity.

Tissue specificity: High expression in the lung, kidney, skeletal muscle and small intestine, and lower level of expression in heart, liver, spleen, stomach and fat. Ref.1

Miscellaneous: Brefeldin A induces dissociation from the Golgi of the beta-COP and presumably the other coatomer subunits

By similarity.Important candidate gene for meat quality and growth traits in animal breeding. May also be useful for the research of muscle development mechanisms.

Sequence similarities: Contains 6 HEAT repeats.