Rabbit anti-Human, Mouse Connexin 59 Polyclonal Antibody | anti-Cx59 antibody

Connexin 59, CT (GJA10, Cx59, Connexin-58, Cx58, Gap Junction alpha-9 Protein, Gap Junction alpha-10 Protein, Connexin-59, GJA9) (PE)

Cat. Num. AAA6470243

• Gene Names: GJA9; CX58; CX59; GJA10
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin 59; Polyclonal Antibody; CT (GJA10; Cx59; Connexin-58; Cx58; Gap Junction alpha-9 Protein; Gap Junction alpha-10 Protein; Connexin-59; GJA9) (PE); anti-Cx59 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJA10. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Cx59 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GJA10 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GJA10 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). GJA10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Cx59 antibody

Antibodies; Connexin; Junction Proteins

References

1. Hombach S, et al., Eur J Neurosci. 2004 May;19(10):2633-40. 2. Houghton FD, et al., Biol Reprod. 2002 May;66(5):1403-12. 3. Manthey D, et al., J Biol Chem. 1999 May 21;274(21):14716-23.

NCBI and Uniprot Product Information

• NCBI GI #: 31542845
• NCBI GeneID: 81025
• NCBI Accession #: NP_110399
• NCBI GenBank Nucleotide #: NM_030772
• UniProt Accession #: P57773
• Molecular Weight: 59kDa
• NCBI Official Full Name: gap junction alpha-9 protein
• NCBI Official Synonym Full Names: gap junction protein alpha 9
• NCBI Official Symbol: GJA9
• NCBI Official Synonym Symbols: CX58; CX59; GJA10
• NCBI Protein Information: gap junction alpha-9 protein
• UniProt Protein Name: Gap junction alpha-9 protein
• UniProt Gene Name: GJA9
• UniProt Synonym Gene Names: GJA10; Cx58; Cx59
• UniProt Entry Name: CXA9_HUMAN

NCBI Description

Connexins, such as GJA9, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]

Uniprot Description

GJA9: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Alpha-type (group II) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: connexon complex; integral to membrane

Biological Process: cell communication