Rabbit anti-Mouse Connexin 46 Polyclonal Antibody | anti-cx28.9 antibody
Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)
Gene Names
cx28.9; gjac; cx20c; fd51f05; zgc:92056; wu:fd51f05; si:dkey-261a18.3
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Connexin 46; Polyclonal Antibody; Connexin 46 (Cx46; Gap Junction Alpha 3 Protein; CXA-3); Anti -Connexin 46 (Cx46; anti-cx28.9 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Connexin 46 protein. Species Sequence Homology: rat, Chinese hamster and Golden hamster.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-cx28.9 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: Western Blot: 1-3ug/ml. Identifies a single band at ~46kD
Immunofluorescence: 3ug/ml
Immunohistochemistry (Frozen): 3ug/ml
Note: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Dilution: Western Blot: 1-3ug/ml. Identifies a single band at ~46kD
Immunofluorescence: 3ug/ml
Immunohistochemistry (Frozen): 3ug/ml
Note: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning.
Immunogen
Synthetic peptide corresponding to an internal region of the mouse Connexin 46 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-cx28.9 antibody
Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46kD protein. Cx46, along with Cx50, is principally expressed in the lens of the eye. Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells. Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function. Mutations of Cx46 result in severe cataracts of the lens.
Product Categories/Family for anti-cx28.9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
connexin 28.9
NCBI Official Synonym Full Names
connexin 28.9
NCBI Official Symbol
cx28.9
NCBI Official Synonym Symbols
gjac; cx20c; fd51f05; zgc:92056; wu:fd51f05; si:dkey-261a18.3
NCBI Protein Information
connexin 28.9; gap junction protein, alpha c
Similar Products
Product Notes
The cx28.9 (Catalog #AAA611717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 46 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Dilution: Western Blot: 1-3ug/ml. Identifies a single band at ~46kD Immunofluorescence: 3ug/ml Immunohistochemistry (Frozen): 3ug/ml Note: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4. Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning. Researchers should empirically determine the suitability of the cx28.9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 46, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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