Rabbit anti-Human Connexin 46 Polyclonal Antibody | anti-Cx46 antibody

Connexin 46, NT (Gap Junction alpha-3 Protein, Connexin-46, Cx46, GJA3) (APC)

Cat. Num. AAA6470212

• Gene Names: GJA3; CX46; CZP3; CTRCT14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Connexin 46; Polyclonal Antibody; NT (Gap Junction alpha-3 Protein; Connexin-46; Cx46; GJA3) (APC); anti-Cx46 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Connexin 46.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Cx46 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acidsaa108-139 near the N-terminal region of human Connexin 46 (NP_068773, Q9Y6H8).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Jurkat cell line lysates (35ug/lane) usingMBS624330. GJA3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using MBS624330 and peroxidase-conjugated secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Cx46 antibody

Antibodies; Connexin; Junction Proteins

References

1. Mackay, D., et al., Am. J. Hum. Genet. 64(5):1357-1364 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 22779877
• NCBI GeneID: 2700
• NCBI Accession #: NP_068773
• NCBI GenBank Nucleotide #: NM_021954
• UniProt Accession #: Q9Y6H8
• Molecular Weight: 47kDa
• NCBI Official Full Name: gap junction alpha-3 protein
• NCBI Official Synonym Full Names: gap junction protein alpha 3
• NCBI Official Symbol: GJA3
• NCBI Official Synonym Symbols: CX46; CZP3; CTRCT14
• NCBI Protein Information: gap junction alpha-3 protein
• UniProt Protein Name: Gap junction alpha-3 protein
• UniProt Gene Name: GJA3
• UniProt Synonym Gene Names: Cx46
• UniProt Entry Name: CXA3_HUMAN

NCBI Description

The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3). [provided by RefSeq, Jan 2010]

Uniprot Description

GJA3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA3 are the cause of cataract zonular pulverulent cataract type 3 (CZP3). A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes. Defects in GJA3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: connexon complex; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: cell-cell signaling; visual perception; transport; transmembrane transport

Disease: Cataract 14, Multiple Types