Rabbit anti-Human Connexin-30 Polyclonal Antibody | anti-Cx30 antibody

Connexin-30, NT (Gap Junction beta-6 Protein, GJB6, Cx30) (MaxLight 405)

Cat. Num. AAA6470073

• Gene Names: GJB6; ED2; EDH; HED; CX30; HED2; DFNA3; ECTD2; DFNA3B; DFNB1B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin-30; Polyclonal Antibody; NT (Gap Junction beta-6 Protein; GJB6; Cx30) (MaxLight 405); anti-Cx30 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJB6.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-Cx30 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GJB6 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-Cx30 antibody

Antibodies; Connexin; Junction Proteins

References

1. Beltramello, M., et al., Biochem. Biophys. Res. Commun. 305(4):1024-1033 (2003). 2. Common, J.E., et al., Biochem. Biophys. Res. Commun. 298(5):651-656 (2002). 3. Smith, F.J., et al., J. Invest. Dermatol. 118(3):530-532 (2002). 4. del Castillo, I., et al., N. Engl. J. Med. 346(4):243-249 (2002). 5. Pallares-Ruiz, N., et al., Eur. J. Hum. Genet. 10(1):72-76 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 34978349
• NCBI GeneID: 10804
• UniProt Accession #: O95452; Q5Q1H9; Q5Q1I0; Q5Q1I1; Q5T5U0; Q8IUP0; B3KQN2
• Molecular Weight: 261
• NCBI Official Full Name: Gap junction beta-6 protein
• NCBI Official Synonym Full Names: gap junction protein, beta 6, 30kDa
• NCBI Official Symbol: GJB6
• NCBI Official Synonym Symbols: ED2; EDH; HED; CX30; HED2; DFNA3; ECTD2; DFNA3B; DFNB1B
• NCBI Protein Information: gap junction beta-6 protein; connexin 30; connexin-30; gap junction protein, beta 6 (connexin 30); ectodermal dysplasia 2, hidrotic (Clouston syndrome)
• UniProt Protein Name: Gap junction beta-6 protein
• UniProt Gene Name: GJB6
• UniProt Synonym Gene Names: Cx30
• UniProt Entry Name: CXB6_HUMAN

NCBI Description

Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form (alpha or beta) of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

GJB6: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily. A connexon is composed of a hexamer of connexins. Interacts with CNST

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: connexon complex; apical plasma membrane; integral to membrane; cytosol

Biological Process: negative regulation of cell proliferation; sensory perception of sound; apoptosis; cell communication; response to lipopolysaccharide; ear morphogenesis; response to electrical stimulus; inner ear development; aging

Disease: Deafness, Autosomal Recessive 1a; Clouston Syndrome; Deafness, Autosomal Recessive 1b; Deafness, X-linked 2; Deafness, Autosomal Dominant 3b