Chicken anti-Human Complement C4B Polyclonal Antibody | anti-C4B antibody

Chicken Polyclonal (IgY) to Human Complement C4B

Cat. Num. AAA242416

• Gene Names: C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified
• Synonyms: Complement C4B; Polyclonal Antibody; Chicken Polyclonal (IgY) to Human Complement C4B; Anti-Complement C4B Antibody IHC-plus; C4B; C4B12; C4BD; C4B1; C4B3; CH; Chido form of C4; CPAMD3; Complement C4-B; CO4; Basic complement C4; C4B2; C4B5; C4F; Complement C4B1a; Complement component 4B; Human Complement C4B; anti-C4B antibody

• Host: Chicken
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Anti-Human Complement Component C4b polyclonal antibody reacts with human C4b. C4 is a 210 KDa trimeric glycoprotein containing three polypeptide chains alpha, beta, and gamma with respective molecular weights of 93KDa, 75KDa and 33 KDa. C4 breaks down in.
• Purity/Purification: Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 171115067

• Applicable Applications for anti-C4B antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: ELISA, Flo (0.1 ug/10E6 cells), IHC-P (20 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified human complement C4b.
• Immunogen: Complement C4B antibody was raised against purified human complement C4b.
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-C4B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 224589818
• NCBI GeneID: 721
• NCBI Accession #: NC_000006.11
• NCBI GenBank Nucleotide #: NC_000006.11
• Molecular Weight: 192,751 Da
• NCBI Official Full Name: Homo sapiens chromosome 6, GRCh37.p13 Primary Assembly
• NCBI Official Synonym Full Names: complement component 4B (Chido blood group)
• NCBI Official Symbol: C4B
• NCBI Official Synonym Symbols: CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• NCBI Protein Information: complement C4-B; Chido form of C4; complement C4B1a; basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
• UniProt Protein Name: Complement C4-B
• Protein Family: Complement C4
• UniProt Gene Name: C4B
• UniProt Synonym Gene Names: CO4; CPAMD3
• UniProt Entry Name: CO4B_HUMAN

NCBI Description

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]

Uniprot Description

C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: complement binding; endopeptidase inhibitor activity; carbohydrate binding

Biological Process: detection of molecule of bacterial origin; regulation of complement activation; innate immune response; opsonization; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4b Deficiency