Goat Complement C3, C3c Polyclonal Antibody | anti-C3 antibody

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, AHUS5, ARMD9, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, Complement Component 3, Complement Factor 3, CPAMD1)

Cat. Num. AAA6009571

• Reactivity: Human, Monkey
• Applications: ELISA
• Purity: Purified; Purified
• Synonyms: Complement C3; C3c; Polyclonal Antibody; C3c (Complement Component C3c; C3/C3c; Acylation Stimulating Protein Cleavage Product; ASP; AHUS5; ARMD9; C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1; Complement Component 3; Complement Factor 3; CPAMD1); Anti -Complement C3; anti-C3 antibody

• Host: Goat
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes monkey C3, C3c. In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of monkey serum or plasma. Species Crossreactivity: Old world monkeys (Cercopithecus, Cynomolgus and Baboon), chimpanzee, human
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

• Applicable Applications for anti-C3 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.; Dilution: ELISA: 1:50-1:500
• Immunogen: C3c isolated and purified from pooled normal rhesus monkey serum.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-C3 antibody

C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is

Product Categories/Family for anti-C3 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 99028969
• NCBI GeneID: 280677
• NCBI Accession #: NP_001035559.2
• NCBI GenBank Nucleotide #: NM_001040469.2
• UniProt Accession #: Q2UVX4; Q2KIZ4
• Molecular Weight: 187,253 Da
• NCBI Official Full Name: complement C3 preproprotein
• NCBI Official Synonym Full Names: complement component 3<
• NCBI Official Symbol: C3
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: ASP
• UniProt Entry Name: CO3_BOVIN

Uniprot Description

Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates

By similarity.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes

By similarity.Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2

By similarity.

Subunit structure: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). C3dg interacts with CR2 (via the N-terminal Sushi domains 1 and 2). Interacts with VSIG4. Interacts (both C3a and ASP) with C5AR2; the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of C5AR2, recruitment of ARRB2 to the cell surface and endocytosis of GRP77

By similarity. Interacts with BHV-1 glycoprotein C. Ref.3

Subcellular location: Secreted.

Post-translational modification: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons

By similarity.Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.