Rabbit anti-Mouse COMP Polyclonal Antibody | anti-COMP antibody

Anti-COMP Antibody, Rabbit Polyclonal

Cat. Num. AAA8103737

• Gene Names: Comp; TSP5
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: COMP; Polyclonal Antibody; Anti-COMP Antibody; Rabbit Polyclonal; COMP Antibody; Rabbit PAb; Antigen Affinity Purified; cartilage oligomeric matrix protein; Anti-TSP5 Antibody; anti-COMP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse COMP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-COMP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse COMP
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse COMP, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-COMP rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:84 kDa Observed band size:94 kDa)

Related Product Information for anti-COMP antibody

Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.

References

Posey KL, et al. (2004) Role of TSP-5/COMP in pseudoachondroplasia. Int J Biochem Cell Biol. 36(6): 1005-12.Chen FH, et al. (2005) Interaction of cartilage oligomeric matrix protein/thrombospondin 5 with aggrecan. J Biol Chem. 282(34): 24591-8.Posey KL, et al. (2008) The role of cartilage oligomeric matrix protein (COMP) in skeletal disease. Curr Drug Targets. 9(10): 869-77.Tan K, et al. (2009) The crystal structure of the signature domain of cartilage oligomeric matrix protein: implications for collagen, glycosaminoglycan and integrin binding. FASEB J. 23(8): 2490-501.

NCBI and Uniprot Product Information

• NCBI GI #: 162287140
• NCBI GeneID: 12845
• NCBI Accession #: NP_057894.2
• NCBI GenBank Nucleotide #: NM_016685.2
• UniProt Accession #: Q9R0G6; G3X8Q4
• Molecular Weight: 82,342 Da
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: Comp
• NCBI Official Synonym Symbols: TSP5
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Complexin
• UniProt Gene Name: Comp
• UniProt Synonym Gene Names: COMP

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8 B3.3|8 34.15 cM

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; collagen binding; extracellular matrix structural constituent; fibronectin binding; heparan sulfate proteoglycan binding; heparin binding; protease binding; protein binding; vitamin D binding

Biological Process: apoptosis; cell adhesion; extracellular matrix organization; limb development; negative regulation of apoptosis