Western Blot (WB)
(Western Blot Analysis: Mouse Liver lysate was resolved by electrophoresis, transferred to membrane and probed with C7510-17J (1:500). Proteins were visualized using a IgG (HRP) Pab Dk xRb and a chemiluminescence detection system. Arrow indicates protein Collagen Type I precursor (~140kD) and mature Collagen Type I (70-90kD).)
Rabbit anti-Mouse Collagen Type I Polyclonal Antibody
Collagen Type I (MaxLight 750)
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, immunoassay, Radioimmunoassay
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Collagen Type I; Polyclonal Antibody; Collagen Type I (MaxLight 750); anti-Collagen Type I antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Collagen Type 1. <0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagen Type I. 1% reactivity with mouse Collagen Type III.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-Collagen Type I antibody
Western Blot (WB), Immunohistochemistry (IHC), Radioimmunoassay (RIA), FLISA
Application Notes
WB: 1:500. Detects Collegen Type 1 on 10ug of mouse liver lysate
IHC: 1:40 for immunofluorescent staining of frozen mouse skin and liver tissues
Applications are based on unconjugated antibody.
IHC: 1:40 for immunofluorescent staining of frozen mouse skin and liver tissues
Applications are based on unconjugated antibody.
Immunogen
Collagen Type 1 extracted and purified from mouse skin.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: Mouse Liver lysate was resolved by electrophoresis, transferred to membrane and probed with C7510-17J (1:500). Proteins were visualized using a IgG (HRP) Pab Dk xRb and a chemiluminescence detection system. Arrow indicates protein Collagen Type I precursor (~140kD) and mature Collagen Type I (70-90kD).)
Western Blot (WB)
(Western Blot Analysis: Mouse Liver lysate was resolved by electrophoresis, transferred to membrane and probed with C7510-17J (1:500). Proteins were visualized using a IgG (HRP) Pab Dk xRb and a chemiluminescence detection system. Arrow indicates protein Collagen Type I precursor (~140kD) and mature Collagen Type I (70-90kD).)
Product Categories/Family for anti-Collagen Type I antibody
References
1. Ramshaw, J A and Werkmeister, J A (1988) Anal. Biochem., 168: 82-7 (1988). 2. Martínez-Salgado, Carlos, et al (2006). Exp Cell Res, 312:2093-106 (2006). 3. Phillips, Roderick J, et al. (2004). J. Clin. Invest., 114: 438-46 (2004).
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Product Notes
The Collagen Type I (Catalog #AAA6469675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type I (MaxLight 750) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Radioimmunoassay (RIA), FLISA. WB: 1:500. Detects Collegen Type 1 on 10ug of mouse liver lysate IHC: 1:40 for immunofluorescent staining of frozen mouse skin and liver tissues Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Collagen Type I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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