Collagen, Rat Type I Polyclonal Antibody
Rat Type I Collagen-Lyophilized
Purity
Purity: >95% as judged by SDS-PAGE analysisPurification: Selective salt precipitation.
Synonyms
Collagen; Rat Type I; Polyclonal Antibody; Rat Type I Collagen-Lyophilized; anti-Collagen; Rat Type I antibody
Host
Rat
Clonality
Polyclonal
Purity/Purification
Purity: >95% as judged by SDS-PAGE analysis
Purification: Selective salt precipitation.
Purification: Selective salt precipitation.
Form/Format
Purified protein
Application Notes
Research Application(s): Type I collagen standard
Antigen for antibody production
Coating material for cell culture studies3
Formation of collagen gels
Antigen for antibody production
Coating material for cell culture studies3
Formation of collagen gels
Source
Rat tail tendon
Characterization
To insure lot-to-lot consistency, each batch of product is tested to conform to characteristics of a standard reference reagent using SDS-PAGE analysis
Preparation and Storage
The purified protein is supplied as 0.25 mg in a lyophilized form.
The collagen may be reconstituted by re-suspended in 500mM acetic acid.
The collagen may be transferred into physiological buffers by dialysis at 2-8 degree C.
Purified collagen may be stored at 2-8 degree C for several months without appreciable loss of activity. For long-term storage, the purified collagen may be aliquoted and stored at -20 degree C. Avoid repeated freezing and thawing.
The collagen may be reconstituted by re-suspended in 500mM acetic acid.
The collagen may be transferred into physiological buffers by dialysis at 2-8 degree C.
Purified collagen may be stored at 2-8 degree C for several months without appreciable loss of activity. For long-term storage, the purified collagen may be aliquoted and stored at -20 degree C. Avoid repeated freezing and thawing.
References
1. Miller, E.J. and R.K. Rhodes. 1982. Meth. Enzymol. 82:33.
2. Miller, E.J. In: Extracellular Matrix Biochemistry, K.A. Piez and A.H. Reddi, eds. Elsevier, New York, 1984. pp. 41-81.
3. Bornstein, M.B. 1958. Laboratory Investigation 7:134.
2. Miller, E.J. In: Extracellular Matrix Biochemistry, K.A. Piez and A.H. Reddi, eds. Elsevier, New York, 1984. pp. 41-81.
3. Bornstein, M.B. 1958. Laboratory Investigation 7:134.
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Product Notes
The Collagen, Rat Type I (Catalog #AAA674530) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. Research Application(s): Type I collagen standard Antigen for antibody production Coating material for cell culture studies3 Formation of collagen gels. Researchers should empirically determine the suitability of the Collagen, Rat Type I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen, Rat Type I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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