Rabbit anti-Human COL4A3BP Polyclonal Antibody | anti-COL4A3BP antibody

COL4A3BP, NT (COL4A3BP, CERT, STARD11, Collagen type IV alpha-3-binding protein, Ceramide transfer protein, Goodpasture antigen-binding protein, START domain-containing protein 11, StAR-related lipid transfer protein 11) (Azide free) (HRP)

Cat. Num. AAA6287695

• Gene Names: CERT1; CERT; GPBP; CERTL; MRD34; STARD11; COL4A3BP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COL4A3BP; Polyclonal Antibody; NT (COL4A3BP; CERT; STARD11; Collagen type IV alpha-3-binding protein; Ceramide transfer protein; Goodpasture antigen-binding protein; START domain-containing protein 11; StAR-related lipid transfer protein 11) (Azide free) (HRP); StAR-related lipid transfer protein 11; anti-COL4A3BP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 598

• Applicable Applications for anti-COL4A3BP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COL4A3BP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-124 amino acids from the N-terminal region of human COL4A3BP.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COL4A3BP Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the COL4A3BP antibody detected the COL4A3BP protein (arrow).)

Related Product Information for anti-COL4A3BP antibody

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-COL4A3BP antibody

Antibodies; Binding Proteins

References

Miralem, T., et al. J. Biol. Chem. 285(17):12551-12558(2010). Kudo, N., et al. J. Mol. Biol. 396(2):245-251(2010). Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009): Tomishige, N., et al. Mol. Biol. Cell 20(1):348-357(2009). Asero, R., et al. Int. Arch. Allergy Immunol. 150(3):271-277(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14165452
• NCBI GeneID: 10087
• NCBI Accession #: NP_112729.1
• NCBI GenBank Nucleotide #: NP_112729.1
• UniProt Accession #: Q9Y5P4
• NCBI Official Full Name: ceramide transfer protein isoform 2
• NCBI Official Synonym Full Names: ceramide transporter 1
• NCBI Official Symbol: CERT1
• NCBI Official Synonym Symbols: CERT; GPBP; CERTL; MRD34; STARD11; COL4A3BP
• NCBI Protein Information: ceramide transfer protein
• UniProt Protein Name: Collagen type IV alpha-3-binding protein
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: COL4A3BP
• UniProt Synonym Gene Names: CERT; STARD11; hCERT; GPBP; StARD11
• UniProt Entry Name: C43BP_HUMAN

NCBI Description

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL4A3BP: a protein with a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. Implicated in human autoimmune diseases via its association with the Goodpasture antigen. Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. GPBP has been reported to have Ser/Thr protein kinase activity towards the Goodpasture (GP) antigen, but lacks a conventional serine/threonine kinase domain. Two human isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.9; Lipid-binding; Kinase, protein; Protein kinase, atypical; ATYPICAL group; COL4A3BP family

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; protein kinase activity

Biological Process: heart morphogenesis; endoplasmic reticulum organization and biogenesis; sphingolipid metabolic process; sphingolipid biosynthetic process; in utero embryonic development; cell morphogenesis; lipid homeostasis; signal transduction; protein amino acid phosphorylation; cell proliferation; muscle contraction; immune response; ceramide metabolic process

Disease: Mental Retardation, Autosomal Dominant 34