Rabbit anti-Human, Mouse COL4A1 Polyclonal Antibody | anti-COL4A1 antibody

COL4A1, NT (Collagen alpha-1(IV) Chain, Arresten)

Cat. Num. AAA623876

• Gene Names: COL4A1; arresten
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: COL4A1; Polyclonal Antibody; NT (Collagen alpha-1(IV) Chain; Arresten); Anti -COL4A1; anti-COL4A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COL4A1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-COL4A1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human COL4A1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot using MBS623876 at 1:1000 dilution Lane 1: A549 whole cell lysate 20ug/lane Lane 2: DU145 whole cell lysate 20ug/lane Lane 3: Hela whole cell lysate 20ug/lane Lane 4: HT-1080 whole cell lysate 20ug/lane. Secondary IgG, (H+L) (HRP) goat x rabbit, Predicted band size : 161kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot using MBS623876 at 1:1000 dilution Lane 1: Hela cell line 35ug/lane Lane 2: human placenta tissue lysate 35ug/lane Secondary: IgG, (H+L) (HRP) goat x rabbit)

Immunofluorescence (IF)

(Immunofluorescence of U251 cells using MBS623876 at 1:25. Secondary: IgG (Alexa Fluor 488) goatx rabbit (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).)

Related Product Information for anti-COL4A1 antibody

COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

Product Categories/Family for anti-COL4A1 antibody

Antibodies; Abs to Collagen

NCBI and Uniprot Product Information

• NCBI GI #: 148536825
• NCBI GeneID: 1282
• NCBI Accession #: NP_001836.2
• NCBI GenBank Nucleotide #: NM_001845.4
• UniProt Accession #: P02462; Q1P9S9; Q5VWF6; Q86X41; Q8NF88; Q9NYC5; A7E2W4; B1AM70; Q14051; Q59F15
• Molecular Weight: 160,615 Da
• NCBI Official Full Name: collagen alpha-1(IV) chain preproprotein
• NCBI Official Synonym Full Names: collagen, type IV, alpha 1
• NCBI Official Symbol: COL4A1
• NCBI Official Synonym Symbols: arresten
• NCBI Protein Information: collagen alpha-1(IV) chain; collagen alpha-1(IV) chain; COL4A1 NC1 domain; OTTHUMP00000018698; OTTHUMP00000194462; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A1
• UniProt Entry Name: CO4A1_HUMAN

NCBI Description

This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq]

Uniprot Description

COL4A1: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH). Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC). The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries. Defects in COL4A1 are a cause of familial porencephaly (POREN1). Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular matrix; endoplasmic reticulum lumen; collagen type IV; extracellular region; basement membrane

Molecular Function: protein binding; platelet-derived growth factor binding; extracellular matrix structural constituent; extracellular matrix constituent conferring elasticity

Biological Process: patterning of blood vessels; receptor-mediated endocytosis; collagen catabolic process; axon guidance; extracellular matrix disassembly; extracellular matrix organization and biogenesis; epithelial cell differentiation; blood vessel morphogenesis; brain development; neuromuscular junction development

Disease: Porencephaly 1; Brain Small Vessel Disease With Or Without Ocular Anomalies; Angiopathy, Hereditary, With Nephropathy, Aneurysms, And Muscle Cramps; Retinal Arteries, Tortuosity Of; Hemorrhage, Intracerebral, Susceptibility To