Goat Col11a2 Polyclonal Antibody | anti-Col11a2 antibody

Goat anti-Col11a2 (aa256-268) Antibody

Cat. Num. AAA423405

• Reactivity: Expected from sequence similarity: Mouse
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Col11a2; Polyclonal Antibody; Goat anti-Col11a2 (aa256-268) Antibody; collagen; type XI; alpha 2; RP23-222K15.2; collagen alpha-2(XI) chain; procollagen; Col11a2 antibody; alpha 2 antibody; RP23-222K15.2 antibody; collagen alpha-2(XI) chain antibody; Col11a2 (aa256-268); anti-Col11a2 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HKPQSQEPQKQYP
• Sequence Length: 1650

• Applicable Applications for anti-Col11a2 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Preliminary experiments gave an approx 80kDa band in lysates of cell line NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 162kDa according to NP_034056.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-HKPQSQEPQKQYP, from the internal region of the protein sequence according to NP_034056.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 6753482
• NCBI GeneID: 12815
• NCBI Accession #: NP_034056.1
• NCBI GenBank Nucleotide #: NM_009926.2
• Molecular Weight: 162,072 Da
• NCBI Official Full Name: collagen alpha-2(XI) chain isoform 2
• NCBI Official Synonym Full Names: collagen, type XI, alpha 2
• NCBI Official Symbol: Col11a2
• NCBI Protein Information: collagen alpha-2(XI) chain
• UniProt Protein Name: Collagen alpha-2(XI) chain
• Protein Family: Collagen
• UniProt Gene Name: Col11a2
• UniProt Entry Name: COBA2_MOUSE

NCBI Description

This gene encodes the alpha-2 subunit of type XI collagen, one of the low abundance fibrillar collagens found in cartilage. The encoded protein, in association with other collagen subunits, forms a heterotrimeric type XI procollagen that may undergo proteolytic processing similar to the alpha-1 subunit. Mice lacking the encoded protein exhibit a mild phenotype similar to nonocular Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED) as well as a nonsyndromic form of deafness called DFNA13. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

COL11A2: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. Defects in COL11A2 are the cause of Stickler syndrome type 3 (STL3). STL3 is an autosomal dominant non- ocular form of Stickler syndrome. Classical Stickler syndrome associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular symptoms are absent in STL3. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A2 are the cause of autosomal recessive otospondylomegaepiphyseal dysplasia (OSMED). OSMED is a skeletal dysplasia accompanied by severe hearing loss. The phenotype overlaps that of autosomal dominant skeletal disorders (Stickler and Marshall syndromes) but can be distinguished by disproportionately short limbs and lack of ocular involvement. Defects in COL11A2 are the cause of Weissenbacher- Zweymueller syndrome (WZS). WZS is an autosomal dominant disorder allelic with STL3 and OSMED. WZS is also referred to as heterozygous OSMED. Defects in COL11A2 are the cause of deafness autosomal dominant type 13 (DFNA13). DFNA13 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in COL11A2 are the cause of deafness autosomal recessive type 53 (DFNB53). Defects in COL11A2 are the cause of fibrochondrogenesis type 2 (FBCG2). A severe skeletal dysplasia characterized by a flat midface, short long bones, short ribs with broad metaphyses, and vertebral bodies that show distinctive hypoplastic posterior ends and rounded anterior ends, giving the vertebral bodies a pinched appearance on lateral radiographic views. The chest is small, causing perinatal respiratory problems which usually, but not always, result in lethality. Affected individuals who survive the neonatal period have high myopia, mild to moderate hearing loss, and severe skeletal dysplasia. Belongs to the fibrillar collagen family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Cellular Component: collagen; collagen type XI; extracellular region; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; metal ion binding

Biological Process: cartilage development; chondrocyte differentiation; collagen fibril organization; ossification; palate development; sensory perception of sound; skeletal development; skeletal morphogenesis; soft palate development; tissue homeostasis