Rabbit anti-Human COG2 Polyclonal Antibody | anti-COG2 antibody

COG2, NT (COG2, LDLC, Conserved oligomeric Golgi complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein) (AP)

Cat. Num. AAA6287515

• Gene Names: COG2; LDLC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COG2; Polyclonal Antibody; NT (COG2; LDLC; Conserved oligomeric Golgi complex subunit 2; Component of oligomeric Golgi complex 2; Low density lipoprotein receptor defect C-complementing protein) (AP); Low density lipoprotein receptor defect C-complementing protein; anti-COG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-COG2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103~132 amino acids from the N-terminal region of human COG2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of COG2 Antibody (N-term) in Hela cell line lysates (35ug/lane). COG2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(COG2 Antibody (N-term) (RB17913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(COG2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-COG2 antibody

COG2 encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes.

Product Categories/Family for anti-COG2 antibody

Antibodies; Complement

References

Edwards, T.L., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (5), 721-735 (2009). Luke, M.M., et al. Stroke 40(2):363-368(2009). Shiffman, D., et al. Arterioscler. Thromb. Vasc. Biol. 28(1):173-179(2008). Sohda, M., et al. Traffic 8(3):270-284(2007). Ungar, D., et al. Trends Cell Biol. 16(2):113-120(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 223029519
• NCBI GeneID: 22796
• NCBI Accession #: NP_001138508.1
• NCBI GenBank Nucleotide #: NM_001145036.1
• UniProt Accession #: Q14746; Q86U99
• Molecular Weight: 83,208 Da
• NCBI Official Full Name: conserved oligomeric Golgi complex subunit 2 isoform 2
• NCBI Official Synonym Full Names: component of oligomeric golgi complex 2
• NCBI Official Symbol: COG2
• NCBI Official Synonym Symbols: LDLC
• NCBI Protein Information: conserved oligomeric Golgi complex subunit 2; COG complex subunit 2; conserved oligomeric Golgi complex protein 2; brefeldin A-sensitive, peripheral Golgi protein; low density lipoprotein receptor defect C complementing; low density lipoprotein receptor d
• UniProt Protein Name: Conserved oligomeric Golgi complex subunit 2
• Protein Family: Conserved oligomeric Golgi complex
• UniProt Gene Name: COG2
• UniProt Synonym Gene Names: LDLC; COG complex subunit 2
• UniProt Entry Name: COG2_HUMAN

NCBI Description

This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes. Mutations of this gene are associated with abnormal glycosylation within the Golgi apparatus. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]

Uniprot Description

COG2: Required for normal Golgi morphology and function. Belongs to the COG2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q42.2

Cellular Component: Golgi membrane; Golgi stack; Golgi transport complex; cytosol

Molecular Function: protein binding; protein complex binding; protein transporter activity

Biological Process: intracellular protein transport; intra-Golgi vesicle-mediated transport; oligosaccharide biosynthetic process; protein amino acid glycosylation; Golgi organization and biogenesis