Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (Phospho-Tyr139) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Rabbit Cofilin 1 Polyclonal Antibody | anti-COFILIN antibody
Cofilin 1 antibody
Gene Names
COFILIN; CFL1
Applications
Immunohistochemistry
Purity
Cofilin 1 antibody was purified by immunoaffinity chromatography.
Synonyms
Cofilin 1; Polyclonal Antibody; Cofilin 1 antibody; Polyclonal Cofilin 1; Anti-Cofilin 1; Cofilin1; Cofilin 1 non muscle n cofilin; Phospho Tyr139; Cofilin -1; CFL1; anti-COFILIN antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Cofilin 1 antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-COFILIN antibody
Immunohistochemistry (IHC)
Application Notes
IHC: 1:50-1:100
Biological Significance
Actin-binding proteins regulate assembly and disassembly of actin filaments. Cofilin, a member of the ADF/cofilin family is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. The protein binds to actin monomers and filaments, G actin and F actin, respectively. Cofilin causes depolymerization at the minus end of filaments, thereby preventing their reassembly. The protein is known to sever actin filaments by creating more positive ends on filament fragments.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Cofilin 1 antibody was raised in Rabbit using the peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (Phospho-Tyr139) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (Phospho-Tyr139) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-COFILIN antibody
Rabbit polyclonal Cofilin 1 antibody
Product Categories/Family for anti-COFILIN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,519 Da
NCBI Official Full Name
cofilin-1
NCBI Official Symbol
COFILIN
NCBI Official Synonym Symbols
CFL1
NCBI Protein Information
cofilin-1
UniProt Protein Name
Cofilin-1
UniProt Gene Name
CFL1
UniProt Entry Name
COF1_PIG
Uniprot Description
Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Nuclear receptor co-regulator
Cellular Component: nuclear matrix; cytoplasm; actin cytoskeleton
Molecular Function: actin binding
Biological Process: actin filament depolymerization; regulation of cell morphogenesis; cytoskeleton organization and biogenesis
Research Articles on COFILIN
Similar Products
Product Notes
The COFILIN cfl1 (Catalog #AAA835175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cofilin 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). IHC: 1:50-1:100. Researchers should empirically determine the suitability of the COFILIN cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cofilin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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