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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit Coenzyme Q10 Homolog B Polyclonal Antibody | anti-COQ10B antibody

Polyclonal Antibody to Coenzyme Q10 Homolog B (COQ10B)

Reactivity
Homo sapiens (Human)
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Synonyms
Coenzyme Q10 Homolog B; Polyclonal Antibody; Polyclonal Antibody to Coenzyme Q10 Homolog B (COQ10B); anti-COQ10B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Isotype
IgG
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/mL (varies by lot)
Applicable Applications for anti-COQ10B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry-Frozen (IHC-f), Immunohistochemistry-Paraffin (IHC-p), Enzyme-Linked Immunoabsorbent Assay
Application Notes
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry-Frozen: 1:50-500
Immunohistochemistry-paraffin: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Immunogen
Recombinant COQ10B (Ala38~Thr238) with two N-terminal Tags, His-tag and GST-tag expressed in E.coli (MBS2029279).
Preparation and Storage
Store at 4°Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.C for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human COQ10B Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human COQ10B Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
coenzyme Q-binding protein COQ10 homolog B, mitochondrial isoform 2
NCBI Official Synonym Full Names
coenzyme Q10B
NCBI Official Symbol
COQ10B
NCBI Protein Information
coenzyme Q-binding protein COQ10 homolog B, mitochondrial
UniProt Protein Name
Coenzyme Q-binding protein COQ10 homolog B, mitochondrial
UniProt Gene Name
COQ10B

Uniprot Description

COQ10B: Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes. Belongs to the COQ10 family.

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function: ubiquinone binding

Biological Process: cellular respiration; ubiquinone biosynthetic process

Research Articles on COQ10B

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Product Notes

The COQ10B coq10b (Catalog #AAA2027546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Coenzyme Q10 Homolog B (COQ10B) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Coenzyme Q10 Homolog B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry-Frozen (IHC-f), Immunohistochemistry-Paraffin (IHC-p), Enzyme-Linked Immunoabsorbent Assay. Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry-Frozen: 1:50-500 Immunohistochemistry-paraffin: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the COQ10B coq10b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coenzyme Q10 Homolog B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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