Rabbit anti-Human, Rat Coagulation Factor XII Polyclonal Antibody | anti-F12 antibody

Coagulation Factor XII (F12, HAF, HAE3, HAEX) (PE)

Cat. Num. AAA6411997

• Gene Names: F12; HAF; HAE3; HAEX
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Coagulation Factor XII; Polyclonal Antibody; Coagulation Factor XII (F12; HAF; HAE3; HAEX) (PE); anti-F12 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes F12. Species Crossreactivity: human, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-F12 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human F12.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using F12 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using F12 antibody at dilution of 1:100 (200x lens).)

Product Categories/Family for anti-F12 antibody

Antibodies; Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 145275213
• NCBI GeneID: 2161
• NCBI Accession #: NP_000496.2
• NCBI GenBank Nucleotide #: NM_000505.3
• UniProt Accession #: P00748; P78339
• Molecular Weight: 67,792 Da
• NCBI Official Full Name: coagulation factor XII
• NCBI Official Synonym Full Names: coagulation factor XII (Hageman factor)
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; HAE3; HAEX
• NCBI Protein Information: coagulation factor XII; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF
• UniProt Entry Name: FA12_HUMAN

NCBI Description

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Uniprot Description

F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.

Protein type: Protease; Chaperone; Secreted, signal peptide; Secreted; EC 3.4.21.38

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity; misfolded protein binding

Biological Process: response to misfolded protein; positive regulation of blood coagulation; proteolysis; protein autoprocessing; fibrinolysis; plasma kallikrein-kinin cascade; innate immune response; positive regulation of fibrinolysis; zymogen activation; protein processing; blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation

Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency