KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: COTL1 knockout Hela cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human COTL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit anti-Human Coactosin Like Protein 1 Polyclonal Antibody | anti-COTL1 antibody
Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)
Gene Names
COTL1; CLP
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Coactosin Like Protein 1; Polyclonal Antibody; Polyclonal Antibody to Coactosin Like Protein 1 (COTL1); anti-COTL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against COTL1. It has beenselected for its ability to recognize COTL1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-COTL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
COTL1 (Met1~Glu142)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant COTL1 (Met1~Glu142) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: COTL1 knockout Hela cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human COTL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: COTL1 knockout Hela cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human COTL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Hela Cells; Lane3: Mouse Placenta Tissue; Lane4: Mouse Kidney Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Hela Cells; Lane3: Mouse Placenta Tissue; Lane4: Mouse Kidney Tissue.)
Product Categories/Family for anti-COTL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,945 Da
NCBI Official Full Name
coactosin-like protein
NCBI Official Synonym Full Names
coactosin like F-actin binding protein 1
NCBI Official Symbol
COTL1
NCBI Official Synonym Symbols
CLP
NCBI Protein Information
coactosin-like protein
UniProt Protein Name
Coactosin-like protein
UniProt Gene Name
COTL1
UniProt Synonym Gene Names
CLP
Similar Products
Product Notes
The COTL1 cotl1 (Catalog #AAA20876) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Coactosin Like Protein 1 (COTL1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Coactosin Like Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the COTL1 cotl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coactosin Like Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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